Table 1: Observed and predicted values of endoglucanase activity for the independent variables SCB and CSL concentrations used in central composite rotational design (CCRD), from the crude enzyme extract of Streptomyces misionensis PESB-25.

RunSCB (%w/v)/Coded levelCSL (%w/v)/Coded levelEndoglucanase activity (U·mL−1) Observed*Endoglucanase activity (U·mL−1) Predicted

10.65 (−1)0.77 (−1)0.95 ± 0.110.91
21.35 (+1)0.77 (−1)0.72 ± 0.0050.67
30.65 (−1)1.63 (+1)0.86 ± 0.0030.85
41.35 (+1)1.63 (+1)0.98 ± 0.0380.96
50.5 (−1.41)1.2 (0)0.93 ± 0.0220.95
61.5 (1.41)1.2 (0)0.83 ± 0.0320.86
71.0 (0)0.6 (−1.41)0.66 ± 0.0030.71
81.0 (0)1.8 (1.41)0.87 ± 0.0240.88
91.0 (0)1.2 (0)1.03 ± 0.0161.01
101.0 (0)1.2 (0)1.03 ± 0.0441.01
111.0 (0)1.2 (0)1.00 ± 0.003 1.01

The statistical analysis of the results was performed using the software Design Expert 7.0 (trial version).
*Values are based on Mean ± SD of 3 individual observations.