Novel One-Step Multiplex PCR-Based Method for HLA Typing and Preimplantational Genetic Diagnosis of -Thalassemia
Figure 4
Profiles of 2 different combinations of markers throughout the HLA region after the update of the method on single blastomeres biopsied from nonrelated embryos. Although the efficiency of the PCR varies depending on the marker, the amplification levels obtained for them are adequate to perform the analysis simultaneously. Appropriate zoom of the -axis of the fluorescence level (proportional to the amplification level), let us to observe and analyze all the peaks, corresponding to the different markers.
