Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa
Figure 5
Determination of kinetic parameters for the interaction of EF-Tu with GDP in the presence of EF-Ts. (a) Initial velocities for P. aeruginosa EF-Tu in GDP exchange reactions were determined at various concentrations of GDP. The concentration of EF-Tu was held constant at 0.64 μM, and the concentration of EF-Ts was held at 0.01 μM. The velocities were measured between 15 and 75 s. The concentrations of GDP were ■, 0.4 μM; ◯, 2 μM; ▴, 4 μM; ◆, 8 μM; ●, 12 μM. (b) The data were used to develop a Lineweaver-Burk plot to determine kinetic parameters for the interaction of P. aeruginosa EF-Tu with GDP in the presence of EF-Ts.
