Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

Table 1

Summary of the Wuzhishan miniature pig BAC library.

Segment	Sample	Cloning enzyme	Total clones	Plate No.	Non-insert clone (%)	Average insert size^a (kb)
1	Blood cells	Hind III	92160	1–240	0.8	164.4
2	Fibroblast cells	Hind III	61440	241–400	2.0	134.2
Total			153600	400	1.5	152.3

Note: Genome coverage 7.68× represented probability of finding a target gene was 99.93%. aDetermined using random clones from ligations.