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Figure 2: Comparative histopathology of EAE spinal cords at different days after immunization. Representative cross-sections of spinal cords (5 μm thick) of EAE mice at different days after immunization were stained with Luxol fast blue (for myelin) and immunohistochemically for CD45 (LCA for inflammatory cells) and CD3 (for T cells). A hemi cord is shown in (f). ((a)–(c)) Day 6 after immunization (before EAE onset). ((d)–(f)) Days 9-10 after immunization (EAE onset). ((g)–(i)) Days 13–15 after immunization (progressive phase), ((j)–(l)) Days 30 after immunization (chronic phase). ((a), (d), (g), (j)) LCA stain. ((b), (e), (h), (k) Luxol fast blue. ((c), (f), (i), (l)) CD3. In comparison to day 6 spinal cords, days 9-10 cords demonstrate an influx of LCA and CD3 positive cells in the white matter. Luxol fast blue stain identifies early demyelinating plaques in the white matter. During the progressive phase, there is persistent LCA immunoreactivity, whereas CD3 is decreased. The size of demyelinating plaques increases. At later times (chronic phase), LCA immunoreactivity decreases, while CD3 increases. Demyelinating plaques increase in number and size. Arrows mark locations of demyelinating plaques.