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BioMed Research International
Volume 2013 (2013), Article ID 591313, 6 pages
http://dx.doi.org/10.1155/2013/591313
Research Article

Aqueous Extract of Annona macroprophyllata: A Potential α-Glucosidase Inhibitor

1Unidad de Biomedicina, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico
2Laboratorio de Neurofarmacología de Productos Naturales de la Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz, Calzada México-Xochimilco 101, Colonia San Lorenzo Huipulco, 14370 México City, DF, Mexico
3Instituto Nacional de Medicina Genómica (INMEGEN), Periférico sur 4809, Arenal Tepepan, Tlalpan, Secretaría de Salud, 14610 México City, DF, Mexico
4Departamento de Ecología y Recursos Naturales Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria Coyoacán, 04510 México City, DF, Mexico

Received 29 April 2013; Accepted 25 July 2013

Academic Editor: Ruth C. R. Meex

Copyright © 2013 F. Brindis et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Annona genus contains plants used in folk medicine for the treatment of diabetes. In the present study, an aqueous extract prepared from Annona macroprophyllata (Annonaceae, also known as A. diversifolia) leaves was evaluated on both the activity of yeast α-glucosidase (an in vitro assay) and sucrose tolerance in Wistar rats. The results have shown that the aqueous extract from A. macroprophyllata inhibits the yeast α-glucosidase with an IC50 = 1.18 mg/mL, in a competitive manner with a = 0.97 mg/mL, a similar value to that of acarbose ( = 0.79 mg/mL). The inhibitory activity of A. macroprophyllata was reinforced by its antihyperglycemic effect, at doses of 100, 300, and 500 mg/kg in rats. Chromatographic analysis identified the flavonoids rutin and isoquercitrin in the most polar fractions of A. macroprophyllata crude extract, suggesting that these flavonoids are part of the active constituents in the plant. Our results support the use of A. macroprophyllata in Mexican folk medicine to control postprandial glycemia in people with diabetes mellitus, involving active constituents of flavonoid nature.