Biochemical Characterization and Pharmacological Properties of New Basic PLA₂ BrTX-I Isolated from Bothrops roedingeri (Roedinger's Lancehead) Mertens, 1942, Snake Venom

Figure 5

(a) PLA₂ activity of Bothrops roedingeri venom and peak 4 (BrTX-I); (b) effect of substrate concentration on the kinetics of BrTX-I (PLA₂) activity. (c) effect of temperature on the PLA₂ activity of BrTX-I; (d) effect of pH on BrTX-I activity; (e) influence of ions (10 mM each) on PLA₂ activity in the absence or presence of 1 mM Ca²⁺. The results of all experiments are the mean ± SE, of three determinations (𝑃<0.05) and (f) amino acid composition of BrTX-I from Bothrops roendingeri snake venom.

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