591470.fig.006a
(a)
591470.fig.006b
(b)
591470.fig.006c
(c)
591470.fig.006d
(d)
Figure 6: (a) Neuromuscular blockade in chick biventer cervicis muscle preparation (BCP), after addition of B. roedingeri whole venom (50 μg/mL), or fraction BII-4 (BrTX-I; 5, 20, 50, and 100 μg/mL). (b) Inhibition of the response to ACh and KCl, after a 120 min incubation with PLA2 BrTX-I of Bothrops roedingeri (5, 20, 50, and 100 μg/mL) in chick biventer cervicis muscle preparation. Each point represents the average from five experiments SEM. compared with control. In (c), a group of five Swiss mice (18–20 g) received an intramuscular (i.m.) injection of BrTX-I (1 to 20 μg in 50 μL of PBS), in the gastrocnemius muscle of mice. (d) CK serum levels after control ( ) or PLA2 BrTX-I injection by the i.m. route ( ) and i.v route ( ). At different times, blood was collected, and serum CK levels were measure. Values are means SEM of five mice at each point.