Figure 6: (a) Neuromuscular blockade in chick biventer cervicis muscle preparation (BCP), after addition of B. roedingeri whole venom (50 μg/mL), or fraction BII-4 (BrTX-I; 5, 20, 50, and 100 μg/mL). (b) Inhibition of the response to ACh and KCl, after a 120 min incubation with PLA2 BrTX-I of Bothrops roedingeri (5, 20, 50, and 100 μg/mL) in chick biventer cervicis muscle preparation. Each point represents the average from five experiments SEM. compared with control. In (c), a group of five Swiss mice (18–20 g) received an intramuscular (i.m.) injection of BrTX-I (1 to 20 μg in 50 μL of PBS), in the gastrocnemius muscle of mice. (d) CK serum levels after control () or PLA2 BrTX-I injection by the i.m. route () and i.v route (). At different times, blood was collected, and serum CK levels were measure. Values are means SEM of five mice at each point.