Figure 7: In (a), time-course of the mice paw oedema induced by selected doses of BrTX-I (1–20 μg). The oedema, which was expressed as the percentage increased in the volume of the treated group to that of the control group at each time interval, was maximal around 2 h and decreased thereafter. Levels of TNF-α, IL-1 and IL-6 ((b), (c), and (d), resp.) in the serum after injection of BrTX-I. Animals were injected i.m. with BrTX-I (1.0 mg/kg) or sterile saline alone (control) in a final volume of 1 mL. TNF-α, IL-1 and IL-6 ((b), (c), and (d), resp.) were quantified by specific ELISA, in serum collected at the indicated time intervals after BrTX-I or saline injection as described in Section 2. Each bar represents mean GSEM of 5 animals. when compared with the corresponding control.