Heavy Water Reduces GFP Expression in Prokaryotic Cell-Free Assays at the Translation Level While Stimulating Its Transcription
Figure 1
(a) Typical GFP expression kinetics in a cell-free system in the presence of D2O (0 to 60%). (b) The GFP synthesis rate (mRNA transcription plus protein translation) is reduced in a deuterated environment. (c) Reduction of GFP synthesis rate results in a smaller yield of GFP when the cell-free synthesis reaction expires after about four to five hours due to degradation of ribosomes.
