Purification and Characterization of Tannin Acyl Hydrolase Produced by Mixed Solid State Fermentation of Wheat Bran and Marigold Flower by Penicillium notatum NCIM 923

Table 1

Purification of tannase from P.  notatum NCIM 923.

Purification stage	Total activity (U)	Total protein (mg)	Specific activity (U/mg)	Purification fold	Yield (%)
Crude extract	2067	1814	1.13	1	100
Concentrated	1733	676	2.56	2.26	83.84
75% ammonium sulphate saturated	1358.5	201.5	6.74	5.96	65.72
Dialyzed sample	267.8	25.75	10.40	9.20	12.96
DEAE cellulose column chromatography	242.8	10.8	22.48	19.89	11.77