Hormonal Receptors in Skeletal Muscles of Dystrophic Mdx Mice

Table 1

RT-PCR analysis of various mRNAs: primes, size of the PCR products and conditions of the PCR reactions.

Gene	Primers sequence	Size of PCR product	Temperature (°C)	Number of cycles	Accession number at http://ncbi.nlm.nih.gov/
Androgen receptor	Sense: TACAACTTTCCGCTGGCTCT; Antisense: CCGGAGTAGTTCTCCATCCA	464	58	30	NM013476.2
Glucocorticoid receptor	Sense: CGAGAGTCCTTGGAGGTCAG; Antisense: GATCCTGCTGCTGAGAAAGG	410	55	24	X13358
Estrogen receptor Alpha	Sense: AATTCTGACAATCGACGCCAG; Antisense: GTGCTTCAACATTCTCCCTCCTC	344	55	30 or 40*	NM007956
Estrogen receptor Beta	Sense: ACAGTCCTGCTGTGATGAAC; Antisense: ACTAGTAACAGGGCTGGCAC	271	55	30 or 40*	U81451
Progesterone receptor	Sense: CCAGCATGTCGTCTGAGAAA; Antisense: AAACACCATCAGGCTCATCC	426	60	40	NM008829
𝛼-Actin	Sense: TGTGATGGTGGGAATGGGTCAG; Antisense: TTGATGTCACGCACGATTTCC	513	55	28	AK151136

*Number of cycles varies with the saturation curve by the cycle of each tissue.