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BioMed Research International
Volume 2013 (2013), Article ID 605831, 7 pages
http://dx.doi.org/10.1155/2013/605831
Research Article

Triterpenes and the Antimycobacterial Activity of Duroia macrophylla Huber (Rubiaceae)

1Bioprospection and Biotechnology Laboratory, National Research Institute of Amazonia (INPA), 69060-001 Manaus, AM, Brazil
2Mycobacterial Laboratory, Federal University Foundation of Rio Grande (FURG), 96200-190 Rio Grande, RS, Brazil
3NMR Laboratory, Department of Chemistry, Federal University of Paraná (UFPR), 81530-900 Curitiba, PR, Brazil

Received 9 September 2012; Revised 17 December 2012; Accepted 1 January 2013

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Daiane Martins et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Duroia macrophylla popularly known as “cabeça-de-urubú,” “apuruí,” or “puruí-grande-da-mata” occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in all dichloromethane and methanol extracts, respectively. Methanol extracts from both branches (1st collection) and leaves (2nd collection) presented hydrolyzed tannins, yet alkaloids were only detected in the dichloromethane and methanol extracts from branches at the 2nd collection. Phenol compounds were found in both dichloromethane extracts’ collections. The action of every extract was assayed against Mycobacterium tuberculosis (RMPr, H37Rv, and INHr strains), showing that the dichloromethane extract from leaves (1st collection) has the major biological activity, with a MIC of 6.25 μg/mL for the INHr strain, 25.0 μg/mL for the RMPr strain, and ≤6.25 μg/mL for the H37Rv strain. The chromatographic fractioning of the dichloromethane extract from leaves (1st collection) yielded the isolation of two triterpenes: oleanolic and ursolic acids, which were identified by NMR analysis and reported for the first time in the Duroia genus.