Differentiation of Induced Pluripotent Stem Cells into Male Germ Cells In Vitro through Embryoid Body Formation and Retinoic Acid or Testosterone Induction

Table 1

Primers for q-PCR analysis of male germ cell markers.

Gene	Forward sequence (5′-3′)	Reverse sequence (5′-3′)
Oct-4	CCCTCTGTTCCCGTCACTG	ACCTCCCTTGCCTTGGCT
Dppa3	TGTCGGTGCTGAAAGACCCTAT	TTTCCTTCGAGCCTTTTTTGTC
Piwil2	TGACCTGTGCATCCCCTTCT	TCCCCACAAGCTTCATATCCA
Tex14	GCGTATCGCAGTCGGCA	CCATGTGCAGCACTGGGA
Stra8	GTTTCCTGCGTGTTCCACAAG	CACCCGAGGCTCAAGCTTC
Dazl	AATGTTCAGTTCATGATGCTGCTC	TGTATGCTTCGGTCCACAGACT
Scp1	CGCTACAACCACATGCTTCG	GGAACGCTGCTTAGATCTCCTC
Scp3	ATGCTTCGAGGGTGTGGG	TTCCACCAGGCACCATCTTT
Msy2	CACCAAGGAGGATGTCTTTGTTC	CCAACACTCCGCAGAAACTTC
Odf2	CTGCCTTGTTAAGGTGTTGATGTC	TCATGGCCTTGAAGGATACCA
ACT	GTGTGCAGCCTGCACCAA	ACTGGCGGTCTTGAAAGCA
Akap3	ACGCCACTTTGACTTTGTAACCA	AAGACACCAATAAGGCTCATTCG
Prm1	AGGTGTAAAAAATACTAGATGCACAGAATAG	TTCAAGATGTGGCGAGATGCT
Gapdh	AGA ACATCATCCCTGCATCC	CACATTGGGGGTAGGAACAC