Research Article

Biochemical, Pharmacological, and Structural Characterization of New Basic Bbil-TX from Bothriopsis bilineata Snake Venom

Figure 7

(a) Edema-forming activity of Bbil-TX in mice. Induction of edema by toxins (0.1, 1, and 5 μg/mL), injected s.c. in the footpad of mice. At various time intervals the increase in footpad volume, as compared to controls, was expressed as percent edema. Each point represents the of four animals. Levels of TNF-α (b), IL-6 (c), and IL-1 (d), in the serum after injection of Bbil-TX. Animals were injected i.m. with Bbil-TX (1.0 mg/kg) or sterile saline alone (control) in a final volume of 100 μL. IL-1 and IL-6 were quantified by specific ELISA, and TNF-α was quantified by cytotoxic activity on L929 cells in serum collected at the indicated time intervals after Bbil-TX or saline injection as described in Materials and Methods. Each bar represents of 7 animals. * when compared with the corresponding control groups.
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(a)
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(b)
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(c)
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(d)