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BioMed Research International
Volume 2013 (2013), Article ID 614721, 12 pages
Genetically Distinct Glossina fuscipes fuscipes Populations in the Lake Kyoga Region of Uganda and Its Relevance for Human African Trypanosomiasis
1Faculty of Science, Gulu University, Loroo Division, Gulu Municpality, Gulu, Uganda
2Department of Ecology and Evolutionary Biology, Yale University, 21 Sachem Street, New Haven, CT 06520, USA
3School of Biological Sciences, Makerere University, Kampala University Rd, Kampala, Uganda
4National Livestock Resources Research Institute, P.O. Box 96, Old Busia Road, Tororo, Uganda
5Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06520, USA
Received 30 April 2013; Accepted 19 August 2013
Academic Editor: Harry P. De Koning
Copyright © 2013 Richard Echodu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Appendix 1: Information on the study site, time of collection and number of flies caught in each site.
Appendix 2: Details of microsatellite markers used in the study.
Appendix 3: Per locus estimates of FIS at 18 microsatellite loci for each sampling locality.
Appendix 4: Mitochondrial haplotype information, including frequencies observed across studied populations. New haplotypes recovered from this study are indicated by *.
Appendix 5: Results of an AMOVA testing for temporal genetic structure in four populations of G. fuscipes sampled in 2008 and also in 2011.
Appendix 6: Fst values for temporal samples calculated on microsatellite data. Non-significant values are in bold.
Appendix 7: Microsatellite-based FST values pairwise comparison between sampling localities of G. f. fuscipes in Uganda.
Appendix 8: Microsatellite-based FST values for pairwise comparisons among the three populations detected using Bayesian clustering.
Appendix 9: Details of all first generation migrants detected by Geneclass 2.0, using Lh, Lh/Lmax and STRUCTURE.
Appendix 10: Comparison of mtDNA clade and microsatellite assignment for each individual where both data types were collected.
Figure 1: Microsatellite allele frequencies observed in seven populations of G. f. 52 fuscipessampled at different time points. Numbers after location code indicate the time interval 53 (in generations) since the first sampling.