Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-B Activation
Figure 4
RT-PCR detection of metabotropic and ionotropic glutamate receptors in isolated astrocytes and pinealocytes. (a) RT-PCR detection of transcripts for mGluR1 (79 bp), mGluR2 (198 bp), mGluR3 (196 bp), mGluR5 (165 bp), and mGluR7 (70 bp) in astrocytes (As), pinealocytes (Pi), and hippocampus (Hi). Molecular weight marker (M) and water (H2O) are presented in lanes 1 and 2, respectively. (b) RT-PCR detection of transcripts for GluA1 (203 bp), GluA2 (155 bp), GluA3 (193 bp), and GluN1 (201 bp) in astrocytes (As), pinealocytes (Pi), and hippocampus (Hi). The molecular weight marker (M) and PCR product generated with water (H2O) are presented in lanes 1 and 2, respectively.
