Modulation of Pineal Melatonin Synthesis by Glutamate Involves Paracrine Interactions between Pinealocytes and Astrocytes through NF-𝜿B Activation

Figure 7

Electrophoretic mobility shift assays for NF𝜅B in isolated astrocytes and pinealocytes. (a) Nuclear proteins (5 μg) were extracted from astrocytes treated with glutamate (Glu) 600 μM. (b) Densitometric analysis (arbitrary units) of the NF-κB band is presented on panel (a). One-way ANOVA, Student’s test, ^∗∗𝑃<0.01 versus control group, mean ± SEM. (c) Nuclear proteins (5 μg) were extracted from pinealocytes treated with Glu 600 μM. (d) Densitometric analysis (arbitrary units) of the NF-κB band is presented in (c). The position of the specific NF-κB/DNA binding complex is indicated. 𝑛=5 for each group, 3 experiments.

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