Comparison of Prostate-Specific Promoters and the Use of PSP-Driven Virotherapy for Prostate Cancer

Figure 6

Conditional oncolytic effects of AdPSAE1 in prostate cancer cells. The design of a prostate-specific conditional replication-competent adenovirus AdPSAE1. The native Ad5 early region 1 (E1) gene that is required for adenoviral replication is replaced by an expression cassette that contains an Ad5 E1 gene under the control of an 860 bp PSA promoter. Human prostate cancer cell lines PPC-1 (a) and LNCaP (b), human bladder cancer cell line RT4 (c), human breast cancer cell line MCF-7 (d), and human glioma cell line 9L (e) were transduced with AdPSAE1 or AdPSAlacZ at moi of 1. Attached viable cells were stained with crystal violet 6 days after viral infection and were compared to the untreated controls.