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BioMed Research International
Volume 2013 (2013), Article ID 626258, 8 pages
http://dx.doi.org/10.1155/2013/626258
Research Article

Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells

1Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China

Received 19 May 2013; Revised 30 June 2013; Accepted 2 July 2013

Academic Editor: Ken-ichi Isobe

Copyright © 2013 Yuhua Gao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dermis-derived mesenchymal stem/progenitor cells (DMS/PCs) were isolated from the skin tissue of 16-day-old chick embryos and then characterized by immunofluorescence and RT-PCR. We found that primary DMS/PCs could be expanded for 15 passages. Expression of β-integrin, CD44, CD71, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 DMS/PCs were successfully induced to differentiate into osteoblasts, adipocytes, and neurocytes. The results indicate the potential for multilineage differentiation of DMS/PCs that may represent an ideal candidate for cellular transplantation therapy.