Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells
Figure 5
Osteogenic differentiation of DMS/PCs. (a) Control cells. (b) After induction in osteogenic medium for 14 days, the cells changed from fusiform to triangular in shape and were positive for alizarin red staining. Calcified nodules increased in number and became larger during induction. After about 14 days, the nodules were observed by alizarin red staining. Cells cultured in complete medium showed no morphological changes and were negative for alizarin red staining (scale bar: 100 μm). (c) After induction for 14 days, RT-PCR revealed the expression of osteoblast-specific genes ALP and OPN in the induced group, whereas these genes were not expressed in the control group. Lane 1: AKP was positive in the inducted group; lane 2: OPN was positive in the inducted group; lane 3: AKP was negative in the control group; lane 4: OPN was negative in the control group; lanes 5 and 6: GAPDH served as the internal control.
