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BioMed Research International
Volume 2013 (2013), Article ID 629235, 7 pages
http://dx.doi.org/10.1155/2013/629235
Research Article

Bisphenol A Modifies the Regulation Exerted by Testosterone on 5α-Reductase Isozymes in Ventral Prostate of Adult Rats

1Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Granada, Avenida de Madrid s/n, 18012 Granada, Spain
2Institute of Neurosciences, Faculty of Medicine, University of Granada, 18012 Granada, Spain
3Department of Pathology, San Cecilio University Hospital and School of Medicine, University of Granada, 18012 Granada, Spain

Received 30 April 2013; Revised 20 June 2013; Accepted 6 July 2013

Academic Editor: S. L. Mowbray

Copyright © 2013 Pilar Sánchez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The development, growth, and function of the prostate gland depend on androgen stimulation. The primary androgen in prostate is 5 -dihydrotestosterone (DHT) which is synthesized from circulating testosterone (T) through the action of 5 -reductase (5 -R). Although 5 -R occurs as five isozymes, only 5 -R1 and 5 -R2 are physiologically involved in steroidogenesis. The endocrine disruptor bisphenol A (BPA) alters sexual organs, including the prostate. Our previous findings indicated that BPA decreased the expression of 5 -R1 and 5 -R2 in rat prostate but also circulating T. Thus, it is unclear whether BPA exerts this effect on 5 -R isozymes by reducing circulating T or by any other mechanism. In this study, we examine the effects of short-term exposure to BPA at doses below 25  g/Kg/d and above 300  g/Kg/d of the TDI on mRNA levels of 5 -R1 and 5 -R2 in prostate of adult castrated rats supplemented with T to achieve constant circulating T levels. mRNA levels were measured by absolute quantitative RT-PCR, T levels by RIA, and DHT levels by ELISA. Our results indicated that in castrated rats treated with T BPA at the two doses studied significantly decreased the mRNA levels of both 5 -R isozymes in a dose-dependent manner without modifications in circulating T.