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BioMed Research International
Volume 2013 (2013), Article ID 630683, 7 pages
http://dx.doi.org/10.1155/2013/630683
Research Article

Bovine Papillomavirus Clastogenic Effect Analyzed in Comet Assay

1Laboratório de Genética, Instituto Butantan, Avenida Vital Brasil, 1500, Butantã, 05503-900 São Paulo, SP, Brazil
2Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 2415 Edifício ICB-III-Cidade Universitária, 05508-900 São Paulo, SP, Brazil
3Programa de Pós-graduação em Biologia Estrutural e Funcional, Universidade Federal de São Paulo, Rua Botucatu, 740, 04023-900 São Paulo, SP, Brazil
4Departamento de Biologia, Universidade Federal da Integração Latino-Americana (UNILA), Avenida Tancredo Neves, 6731 bloco 4, 85867-970 Foz do Iguaçú, PR, Brazil

Received 26 March 2013; Accepted 8 May 2013

Academic Editor: Franco Roperto

Copyright © 2013 R. P. Araldi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bovine papillomavirus (BPV) is an oncogenic virus related to serious livestock diseases. Oncoproteins encoded by BPV are involved in several steps of cellular transformation and have been reported as presenting clastogenic effects in peripheral lymphocytes and primary culture cells. The aim of this study was to evaluate the clastogenic potential of BPV types 1, 2, and 4 by comet assay. Peripheral blood was collected from 37 bovines, 32 infected with different levels of papillomatosis (12 animals have no affection) and five calves, virus free (negative control). The viral identification showed presence of more than one virus type in 59.375% of the infected animals. Comet assay was performed according to alkaline technique. The Kruskal-Wallis test showed statistical difference between the negative control group and infected animals ( ). The Dunn post hoc test showed difference comparing the infected animals with calves. Mann-Whitney test verified no difference between animals infected with only one viral type and animals presenting more than one viral type. The comet assay is considered an efficient tool for assessment of damage in the host chromatin due to viral action, specifically highlighting viral activity in blood cells.