Review Article

Role of the Microenvironment in Ovarian Cancer Stem Cell Maintenance

Figure 1

Ovarian cancer cells. (a) Ovarian cancer cell lines, SKOV3 in spheroid culture. Scale bar 100  m. (b) Confocal imaging of an SKOV3 sphere. Scale bar 20  m. (c) Coculture of endothelial cells- (ECs-) GFP (green) and SKOV3 (red). ECs secrete microparticles (arrows) which are uptaken by ovarian cancer cells. Scale bar 10  m. (d) Microparticles from ECs were tagged with WGA-alexa fluor 594 and added to culture of SKOV3 during 6 hours. Ovarian cancer cells are able to uptake ECsmicroparticles. Scale bar 5  m. (e)-(f) Coculture of ECs-GFP (green) and SKOV3 (red). Both cell types are interconnected by tunneling nanotubes. Scale bar 10  m.
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