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Figure 4: Differences in mineralization-associated gene expression patterns in cocultures of periodontal progenitors and centrifuged blood derivatives. (a), (c), and (e) are real-time RT-PCR assays for the osteoblast transcription factor Runx2, and (b), (c), and (f) are real-time RT-PCR assays for the calcification inhibitor Matrix Gla Protein (MGP). Different coculture conditions (PRF, PPP, and DMEM) are distinguished by different bar patterns identified in the upper right corner of the figure. The three periodontal progenitor populations compared in this study, dental follicle (DF), periodontal ligament (PDL), and alveolar bone (AB), are labeled on the -axis of the graphs in (a)–(f). (g) is a western blot comparing Runx2 protein levels generated by AB cells cocultured either with PRF, OM, PPP, or DMEM. GAPDH served as a control. (h) is the corresponding densitometry evaluation. Level of significance was calculated in comparison to the DMEM-treated cells within each group. , , and .