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BioMed Research International
Volume 2013 (2013), Article ID 639725, 5 pages
Research Article

Green Synthesis of Silver Nanoparticles Using Pinus eldarica Bark Extract

Department of Pharmacognosy and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81744-176, Iran

Received 24 April 2013; Accepted 6 August 2013

Academic Editor: Maria Alice Zarur Coelho

Copyright © 2013 Siavash Iravani and Behzad Zolfaghari. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. The objectives of this study were production of silver nanoparticles using Pinus eldarica bark extract and optimization of the biosynthesis process. The effects of quantity of extract, substrate concentration, temperature, and pH on the formation of silver nanoparticles are studied. TEM images showed that biosynthesized silver nanoparticles (approximately in the range of 10–40 nm) were predominantly spherical in shape. The preparation of nano-structured silver particles using P. eldarica bark extract provides an environmentally friendly option, as compared to currently available chemical and/or physical methods.