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BioMed Research International
Volume 2013 (2013), Article ID 640682, 5 pages
http://dx.doi.org/10.1155/2013/640682
Research Article

Modulation of the Antibiotic Activity by Extracts from Amburana cearensis A. C. Smith and Anadenanthera macrocarpa (Benth.) Brenan

1Faculdade Leão Sampaio, 63100-000, Juazeiro do Norte, CE, Brazil
2Faculdade de Medicina Estácio de Juazeiro do Norte, 63100-000, Juazerio de Norte, CE, Brazil
3Laboratório de Pesquisa em Produtos Naturais, Universidade Regional do Cariri, 63105-000, Crato, CE, Brazil
4Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000, Crato, CE, Brazil
5Departamento de Química Biológica, Universidade Regional do Cariri, 63105-000, Crato, CE, Brazil
6Laboratório de Farmacologia e Química Molecular, Universidade Regional do Cariri, 63105-000, Crato, CE, Brazil

Received 15 August 2012; Revised 15 October 2012; Accepted 15 October 2012

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Fernando G. Figueredo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to verify the possible interactions between ethanol extracts of Amburana cearensis A. C. Smith and Anadenanthera macrocarpa (Benth.) Brenan, combined with six antimicrobial drugs against multiresistant strains of Staphylococcus aureus and Escherichia coli isolated from humans. The antibacterial activity of the extracts was determined using the minimum inhibitory concentration (MIC). The microdilution assay was performed to verify the interactions between the natural products and the antibiotics using a subinhibitory concentration. The activity of amikacin associated with the extract of Anadenanthera macrocarpa against EC 27 was enhanced, demonstrating an MIC reduction from 128 to 4 μg/mL. Among the β-lactams, no potentiation on its activity was observed, with exception to the antagonism of the natural products with ampicillin against S. aureus 358.