Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

Table 1

The 10 peritoneal dialysate proteins identified with higher confidence levels (at least three unique peptide sequences matched) in this study.

Accession no.	Protein name	Expression	MW (Da)	PI Value	Mascot score	Match queries	Sequence coverage	Peptide
P02768	Albumin (ALB)	Downregulation	69367	8.2	66	4	17%	K.FQNALLVR.Y
								K.AVMDDFAAFVEK.C + oxidation (M)
								K.KVPQVSTPTLVEVSR.N
P02760	Alpha-1-microglobulin/bikunin preproprotein (AMBP)	Downregulation	38999	5.95	349	7	21%	R.ETLLQDFR.V
								K.WYNLAIGSTCPWLK.K + carbamidomethyl (C);
								R.GECVPGEQEPEPILIPR.V + carbamidomethyl (C)
								R.EYCGVPGDGDEELLRFSN.-
								R.VVAQGVGIPEDSIFTMADR.G
								R.VVAQGVGIPEDSIFTMADR.G + oxidation (M)
								R.ETLLQDFRVVAQGVGIPEDSIFTMADR.G + deamidated (NQ)
P02647	Apolipoprotein A-I (APOA1)	Downregulation	30778	5.39	244	12	44%	K.VQPYLDDFQK.K
								K.WQEEMELYR.Q + oxidation (M)
								R.THLAPYSDELR.Q
								K.VQPYLDDFQKK.W
								R.DYVSQFEGSALGK.Q
								R.VKDLATVYVDVLK.D
								K.VEPLRAELQEGAR.Q + deamidated (NQ)
								K.VEPLRAELQEGAR.Q
								K.LLDNWDSVTSTFSK.L
								R.QKVEPLRAELQEGAR.Q + Gln->pyro-Glu (N-term Q)
								R.QKVEPLRAELQEGAR.Q
								K.DSGRDYVSQFEGSALGK.Q
P01857	Immunoglobulin G1 Fc fragment (IGHG1)	Downregulation	36106	6.95	154	5	36%	K.NQVSLTCLVK.G + carbamidomethyl (C)
								K.FNWYVDGVEVHNAK.T
								R.EPQVYTLPPSRDELTK.N + deamidated (NQ)
								R.TPEVTCVVVDVSHEDPEVK.F + carbamidomethyl (C)
								R.WQQGNVFSCSVMHEALHNHYTQK.S + carbamidomethyl (C); deamidated (NQ)
P02753	Mutant retinol binding protein (RBP4)	Downregulation	23010	6.29	235	4	84%	-.FSGTWYAMAK.K
								-.FSGTWYAMAK.K + oxidation (M)
								K.KDPEGLFLQDNNVAEFSVDETGQMSATAK.G
								K.KDPEGLFLQDNNVAEFSVDETGQMSATAK.G + oxidation (M)
P00738	Haptoglobin alpha2 (HP)	Downregulation	45205	6.46	77	4	21%	K.LPECEADDGCPKPPEIAHGYVEHSVR.Y + 2 carbamidomethyl (C)
								K.AVGDKLPECEADDGCPKPPEIAHGYVEHSVR.Y + 2 carbamidomethyl (C)
								K.LPECEADDGQPPPKCIAHGYVEHSVR.Y + 2 carbamidomethyl (C); deamidated (NQ)
								K.AVGDKLPECEADDGQPPPKCIAHGYVEHSVR.Y + 2 carbamidomethyl (C); deamidated (NQ)
P06727	Apolipoprotein A-IV (APOA4)	Upregulation	45399	5.23	1109	19	43%	K.VNSFFSTFK.E
								R.LTPYADEFK.V
								R.LEPYADQLR.T
								K.ALVQQMEQLR.Q
								R.LTPYADEFKVK.I
								K.LVPFATELHER.L
								R.DKVNSFFSTFK.E
								K.LGEVNTYAGDLQK.K
								K.KLVPFATELHER.L
								K.VKIDQTVEELRR.S
								K.LNHQLEGLTFQMK.K
								K.LKEEIGKELEELR.A
								K.SELTQQLNALFQDK.L
								K.LGPHAGDVEGHLSFLEK.D
								K.SLAELGGHLDQQVEEFR.R
								R.ENADSLQASLRPHADELK.A
								R.QKLGPHAGDVEGHLSFLEK.D
								R.ENADSLQASLRPHADELKAK.I
P25311	Zn-alpha2-glycoprotein (AZGP1)	Upregulation	34259	5.71	739	24	48%	K.SQPMGLWR.Q
								K.CLAYDFYPGK.I + carbamidomethyl (C)
								K.WEAEPVYVQR.A
								K.AREDIFMETLK.D
								K.AYLEEECPATLR.K + carbamidomethyl (C)
								K.QKWEAEPVYVQR.A + Gln->pyro-Glu (N-term Q)
								K.QKWEAEPVYVQR.A
								K.QKWEAEPVYVQR.A + deamidated (NQ)
								K.AYLEEECPATLRK.Y + carbamidomethyl (C)
								K.YYYDGKDYIEFNK.E
								R.QDPPSVVVTSHQAPGEK.K + Gln->pyro-Glu (N-term Q)
								R.AKAYLEEECPATLRK.Y + carbamidomethyl (C)
								K.EIPAWVPFDPAAQITK.Q
								R.QDPPSVVVTSHQAPGEKK.K + Gln->pyro-Glu (N-term Q)
								R.QVEGMEDWKQDSQLQK.A + Gln->pyro-Glu (N-term Q)
								R.QVEGMEDWKQDSQLQK.A + deamidated (NQ); Gln->pyro-Glu (N-term Q); oxidation
								R.QVEGMEDWKQDSQLQK.A
								R.QVEGMEDWKQDSQLQK.A + deamidated (NQ)
								R.QVEGMEDWKQDSQLQK.A + oxidation (M)
								R.QVEGMEDWKQDSQLQK.A + deamidated (NQ); oxidation (M)
								K.NILDRQDPPSVVVTSHQAPGEK.K
								K.NILDRQDPPSVVVTSHQAPGEK.K + deamidated (NQ)
								K.HVEDVPAFQALGSLNDLQFFR.Y
								K.NILDRQDPPSVVVTSHQAPGEKK.K
Q04637	eukaryotic translation initiation factor 4A isoform 1 (EIF4G1)	Upregulation	175491	5.32	469	8	28%	R.QFYINVER.E
							K.GYDVIAQAQSGTGK.T
								K.GVAINMVTEEDKR.T
								K.MFVLDEADEMLSR.G
								R.DFTVSAMHGDMDQK.E
								K.LQMEAPHIIVGTPGR.V
								R.GIYAYGFEKPSAIQQR.A
								K.LNSNTQVVLLSATMPSDVLEVTK.K
P01892	Human class I histocompatibility antigen (HLA-A)	Upregulation	40922	6.46	129	3	36%	R.VNHVTLSQPKIVK.W
							K.SNFLNCYVSGFHPSDIEVDLLK.N + carbamidomethyl (C)
								K.SNFLNCYVSGFHPSDIEVDLLK.N + carbamidomethyl (C); deamidated (NQ)