Thermal Stability of Glucokinases in Thermoanaerobacter tengcongensis

Table 1

Biochemical and kinetic properties of kinases of ROKs in T. tengcongensis.

Parameter	pH optimum	Apparent 𝑇opt (°C)	Apparent 𝐾𝑚 (mM)		Apparent 𝑉max (U/mg)		Sugar specificity (𝑉max%)				Cation specificity (𝑉max%)		Phosphoryl donor specificity (𝑉max%)
			Glucose/gluNac	ATP/ADP	Glucose/gluNac	ATP/ADP	2-deoxyglucose	Fructose, galactose	Glucose, mannose	galNac, gluNac, manNac	Ca^2+, Cu^2+, Mg^2+	Ni^2+, Sr^2+, Zn^2+	ADP, ATP, CTP	GTP, ITP, PEP
TTE0090	8.0	75	2.4/ND	2.6/ND	90/ND	100/ND	13.5	3, 0	100, 99	0, 0, 0	13.2, 0.4, 100	69.1, 17, 34	3, 100, 38	96, 87, 0
TTE1961	8.0	80	2.1/ND	ND/0.9	93/ND	ND/106	1.2	0, 0	100, 17	0, 0, 0	ND, ND, 100	ND, ND, ND	100, 0, ND	ND, ND, ND
TTE2418	7.5	70	ND/0.9	1.9/ND	ND/229	51/ND	0	0, 6.5	23.9, 6.5	0, 100, 99.8	2.6, 0, 100	5.2, 3.5, 11.3	7, 100, 7	15.5, 57.7, 0