Cultivation-Dependant Assessment, Diversity, and Ecology of Haloalkaliphilic Bacteria in Arid Saline Systems of Southern Tunisia
Figure 2
Phylogenetic diversity of haloalkaliphilic bacteria. (a) Unrooted phylogenetic tree of 44 partial 16S rRNA sequences (500 bp) of the arid saline system isolates with the 24 closest phylogenetic relatives. The method of Jukes and Cantor was used to calculate evolutionary distances and tree topology was constructed using MEGA 4.0. Bootstrap values ( replicates) were indicated at the nodes. The number of isolates per ITS haplotype, the 16S rRNA similarity percentage (refseq rna database), and NaCl range for growth at pH 11 (or at pH 7 where mentioned), are indicated in parenthesis. (b) 16S–23S rRNA ITS haplotypes of 44 representative isolates as resolved on 2% agarose gels. ITS haplotype numbers are indicated. Lane M corresponds to a 100 bp ladder.
