Research Article

Cultivation-Dependant Assessment, Diversity, and Ecology of Haloalkaliphilic Bacteria in Arid Saline Systems of Southern Tunisia

Table 1

Phylogenetic characterization of the haloalkaliphiles (ITS haplotype and 16S rRNA identification) and samples (code, nature, and location) collected from arid saline systems in southern Tunisia during February 2008 and 2010.

Representative strains of ITS haplotypes
(number of isolates per haplotype)
Accession
number
Phylogenetic
group
Closet described species
and identity (%)
Accession
number
Sampling
date
Sample
code
Sampling sites
(number of isolates per site)
Type of
matrix

H1-BMG G12 (1)KF179184Proteobacteria Halomonas boliviensis (98%)EU_308325.1February 2010BDV1.8.A Ksar GhilaneMud
H2-BMG F5 (2)KF179185FirmicutesBacillus saliphilus (96%)HM_811185.1February 2010BDV1.8.BKsar GhilaneBlack sediment
H3-BMG D30 (3)KF179190Proteobacteria Halomonas janggokensis (100%)EU_308361.1February 2010BDV1.8.CKsar GhilaneThermomineral water
H4-BMG D39 (3)KF179189FirmicutesBacillus saliphilus (95%)HM_811185.1February 2010BDV1.8.CKsar GhilaneThermomineral water
H5-BMG D32 (3)KF179187ActinobacteriaNesterenkonia lacusekhoensis (98%)GQ_064877.1February 2010BDV1.4
BDV6.2
Ksar Ghilane (1) 
Chottel Douz (2)
Sand with vegetation
Salt crust
H6-BMG G3 (1)KF179188FirmicutesBacillus saliphilus (95%)HM_811185.1February 2010BDV1.8.BKsar GhilaneBlack sediment
H7-BMG F7 (1)KF179205Proteobacteria Halomonas gomseomensis (99%)EU_308352.1February 2010BDV4.2 Sabkhet EnnaouelSaline water
H8-BMG ED18 (4)KF179213FirmicutesSalinicoccus alkaliphilus (98%)GU_363531.1February 2008BDV6.3Chottel Douz Algal biofilm
H9-BMG D26 (6)KF179191Proteobacteria Halomonas janggokensis (100%)EU_308361.1February 2010BDV1.8.A
BDV6.3
Ksar Ghilane (1) 
Chottel Douz (5)
Mud
Algal biofilm
H10-BMG F8 (3)KF179206Proteobacteria Halomonas taeanensis (98%)HQ_190038February 2010BDV4.1
BDV17.3/18.3
Sabkhet Ennaouel (1) 
Chottel Djerid (2)
Sediment with salt crust
Sediment with salt
H11-BMG G6 (1)KF179174Proteobacteria Halomonas elongata (99%)NR_029227.1February 2010BDV18.3Chottel DjeridBlack sediment
H12-BMG ED65 (7)KF179199FirmicutesSalimicrobium luteum (100%)FJ_157154.1February 2008BDIII-11.A1/B1
BDV6.2
Sabkhet El Melah (6) 
Chottel Douz (1)
Sediment with salt
Salt crust
H13-BMG ED6 (3)KF179211FirmicutesOceanobacillus iheyensis (98%)GU_326361.1February 2008BDV6.2Chottel Douz Salt crust
H14-BMG F4 (1)KF179175ActinobacteriaNesterenkonia halobia (99%)EF_153433.1February 2010BDV20.1Chottel DjeridSand
H15-BMG E7 (1)KF179194FirmicutesSalinicoccus hispanicus (99%)NR_025645.1February 2008BDIII-11.C3Sabkhet El MelahSalicornia rhizosphere
H16-BMG E6 (1)KF179210FirmicutesSalinicoccus alkaliphilus (97%)GU_363531.1February 2008BDV6.3Chottel DouzAlgal biofilm
H17-BMG ED88 (11)KF179193FirmicutesSalinicoccus hispanicus (99%)NR_025645.1February 2008BDIII-11.C3
BDV6.3/BDV6.2
Sabkhet El Melah (7) 
Chottel Douz (4)
Salicornia rhizosphere
Algal biofilm/salt crust
H18-BMG G2 (1)KF179183FirmicutesPiscibacillus salipiscarius (98%)HM_222702.1February 2010BDV19.6Chottel DjeridSediment and salt
H19-BMG E11 (4)KF179202FirmicutesHalobacillus litoralis (100%)HM_636928.1February 2008BDIII-11.B1/A2
BDV20.2
Sabkhet El Melah (3) 
Chottel Djerid (1)
Sediment with salt crust
Sediment (sandy soil)
H20-BMG D102 (1)KF179176Proteobacteria Halomonas elongata (99%)NR_029227.1February 2010BDV18.3Chottel DjeridSediment
H21-BMG ED25 (1)KF179200ActinobacteriaArthrobacter gangotriensis (99%)FR_749771.1February 2008BDIII-11.A1Sabkhet El MelahSediment with salt
H22-BMG D91 (5)KF179178Proteobacteria Halomonas janggokensis (100%)EU_308361.1February 2010BDV17.2
BDV1.8.A/1.8.C
Chottel Djerid (1) 
Ksar Ghilane (4)
Salt crust
Mud/thermomineral water
H23-BMG ED46 (2)KF179192FirmicutesSalinicoccus hispanicus (99%)NR_025645.1February 2008BDIII-11.C3
BDV6.3
Sabkhet El Melah (1) 
Chottel Douz (1)
Salicornia rhizosphere
Algal biofilm
H24-BMG D115 (15)KF179177ActinobacteriaNesterenkonia halobia (99%)EF_153433.1February 2010BDV20.1/19.6
BDV1.4
BDV4.2/4.1
Chottel Djerid (4) 
Ksar Ghilane (7) 
Sabkhet Ennaouel (4)
Sand
Sand with vegetation
Saline water/salt crust
H25-BMG ED60 (2)KF179208FirmicutesSalinicoccus hispanicus (99%)NR_025645.1February 2008BDV6.3
BDIII-11.C3
Chottel Douz (1) 
Sabkhet El Melah (1)
Algal biofilm
Salicornia rhizosphere
H26-BMG G7 (2)KF179182Proteobacteria Halomonas elongata (99%)NR_029227.1February 2010BDV20.2Chottel Djerid Sediment
H27-BMG E9 (6)KF179209FirmicutesOceanobacillus iheyensis (98%)HM_854234.1February 2008BDV6.2Chottel DouzSalt crust
H28-BMG G8 (1)KF307740ActinobacteriaLeucobacter chromiireducens (99%)EF_153433.1February 2008BDIII-11.A2Sabkhet El MelahSediment with water and salt crust
H29-BMG E4 (1)KF179215Proteobacteria Halomonas ventosae (99%)FM_210950.1February 2008BDV6.1Chottel DouzSaline water
H30-BMG G11 (1)KF179180Proteobacteria Halomonas subterranea (99%)EU_308353.1February 2010BDV17.3Chottel DjeridSediment
H31-BMG ED15 (3)KF179214FirmicutesOceanobacillus picturae (98%)HM_179167.1February 2008BDV6.3
BDIII-11.B1
Chottel Douz (2) 
Sabkhet El Melah (1)
Algal biofilm
Sediment with salt
H32-BMG E2 (2)KF179198FirmicutesBrevibacillus agri (99%)HM_355618.1February 2008BDIII-11.A1Sabkhet El MelahSediment with salt
H33-BMG E1 (1)KF179203FirmicutesHalobacillus litoralis (100%)HM_636928.1February 2008BDIII-11.A2Sabkhet El MelahSediment with water and salt crust
H34-BMG E5 (2)KF179197FirmicutesPiscibacillus salipiscarius (98%)HM_222702.1February 2008BDIII-11.A1Sabkhet El MelahSediment with salt
H35-BMG ED37 (1)KF179195FirmicutesSalinicoccus hispanicus (99%)NR_025645.1February 2008BDIII-11.C3Sabkhet El MelahSalicornia rhizosphere
H36-BMG D109 (1)KF179179FirmicutesHalobacillus profundi (99%)EU_482426.1February 2010BDV19.6Chottel DjeridSediment and salt
H37-BMG E3 (1)KF179207Proteobacteria Halomonas ventosae (99%)FM_210950.1February 2008BDV6.1Chottel DouzSaline water
H38-BMG G4 (2)KF179181FirmicutesHalobacillus litoralis (100%)HM_636928.1February 2010BDV17.3Chottel DjeridSediment
H39-BMG F11 (1)KF179186Proteobacteria Halomonas ventosae (99%)AB_617544.1February 2010BDV1.8.BKsar GhilaneBlack sediment
H40-BMG D16 (1)KF179204Proteobacteria Halomonas gomseomensis (99%)EU_308352.1February 2010BDV4.2Sabkhet EnnaouelSaline water
H41-BMG E8 (3)KF307741FirmicutesMarinococcus halophilus (99%)FJ_887949.1February 2008BDIII-11.C3Sabkhet El MelahSalicornia rhizosphere
H42-BMG F2 (1)KF179201Proteobacteria Halomonas boliviensis (98%)EU_308325.1February 2008BDIII-11.C3Sabkhet El MelahSalicornia rhizosphere
H43-BMG ED33 (8)KF179196FirmicutesVirgibacillus  halodenitrificans (99%)AM_950296.1February 2008BDIII-11.B1/C3Sabkhet El Melah (6) Sediment with salt crust/salicornia rhizosphere
BDV6.2Chottel Douz (2)Salt crust
H44-BMG D12 (1)KF179212FirmicutesOceanobacillus iheyensis (98%)HM_854234.1February 2008BDV6.2Chottel DouzSalt crust