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BioMed Research International
Volume 2013 (2013), Article ID 651945, 11 pages
http://dx.doi.org/10.1155/2013/651945
Research Article

Modification of Decellularized Goat-Lung Scaffold with Chitosan/Nanohydroxyapatite Composite for Bone Tissue Engineering Applications

1Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, Saharanpur Campus, Saharanpur, UP 247001, India
2Department of Pathology, All India Institute of Medical Sciences, New Delhi 110029, India
3Department of Molecular Medicine & Biology, Jaslok Hospital and Research Center, Mumbai, Maharashtra 400026, India

Received 2 April 2013; Accepted 26 May 2013

Academic Editor: Ulrich Kneser

Copyright © 2013 Sweta K. Gupta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Decellularized goat-lung scaffold was fabricated by removing cells from cadaver goat-lung tissue, and the scaffold was modified with chitosan/nanohydroxyapatite composite for the purpose of bone tissue engineering applications. MTT assay with osteoblasts, seeded over the chitosan/nanohydroxyapatite-modified decellularized scaffold, demonstrated significantly higher cell growth as compared to the decellularized scaffold without modification. SEM analysis of cell-seeded scaffold, after incubation for 7 days, represented a good cell adhesion, and the cells spread over the chitosan/nanohydroxyapatite-modified decellularized scaffold. Expression of bone-tissue-specific osteocalcin gene in the osteoblast cells grown over the chitosan/nanohydroxyapatite-modified decellularized scaffold clearly signifies that the cells maintained their osteoblastic phenotype with the chitosan/nanohydroxyapatite-modified decellularized scaffold. Therefore, it can be concluded that the decellularized goat-lung scaffold-modified with chitosan/nanohydroxyapatite composite, may provide enhanced osteogenic potential when used as a scaffold for bone tissue engineering.