Figure 1: (a) Scanning electron micrograph of decellularized goat-lung tissue at 250X showing porous architecture. (b) DNA content in native lung tissue and the decellularized tissue. Native tissue served as a positive control. (c) Hematoxylin and eosin staining of decellularized lung tissue section showing collagenous protein structure with negative cell nuclei staining. No nuclear counterstaining was detectable, indicating a total cell removal. (d) EDS spectrum of the decellularized scaffold, showing carbon and oxygen as the major constituents of scaffold, confirming its organic nature. ( ) stands for significant difference between native lung tissue and decellularized tissue ( ).