Figure 1: (a) Scanning electron micrograph of decellularized goat-lung tissue at 250X showing porous architecture. (b) DNA content in native lung tissue and the decellularized tissue. Native tissue served as a positive control. (c) Hematoxylin and eosin staining of decellularized lung tissue section showing collagenous protein structure with negative cell nuclei staining. No nuclear counterstaining was detectable, indicating a total cell removal. (d) EDS spectrum of the decellularized scaffold, showing carbon and oxygen as the major constituents of scaffold, confirming its organic nature. () stands for significant difference between native lung tissue and decellularized tissue ().