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654354.fig.005b
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Figure 5: (a) Computerized image analysis verified results from immunohistochemistry analysis demonstrating a statistically significant increased expression of EGFR in patients with fibrotic forms of IIPs compared to the inflammatory component of cellular NSIP as well as control lung samples. * (b) computerized image analysis also revealed a statistically significant increased expression of EGFR in the alveolar epithelium in IPF, COP, and fNSIP samples compared to cNSIP and normal alveolar epithelium. (c) Quantitative real-time PCR revealed a statistically significant upregulation of Egfr mRNA levels in patients with the fibrotic forms of IIPs including IPF, COP, and fibrotic NSIP compared to cellular NSIP and control lung samples. All values were normalized with the reference gene B2m and presented as relative expression to the control sample as described in materials and methods. * (d) quantitative real-time PCR revealed a statistically significant upregulation of COL1A2 mRNA levels in patients with the fibrotic forms of IIPs including IPF, COP, and fibrotic NSIP compared to cellular NSIP and control lung samples. All values were normalized with the reference gene actin and presented as relative expression to the control sample as described in materials and methods. * .