Figure 3: Level of HSPA8 mRNA 24 h after transfection of artificial box C/D RNAs into MCF-7 cells (160 nM RNA in culture medium). MCF-7 human cells were transfected with RNA7, RNA8, RNA9, RNA10, RNA11, RNA12, RNA7D*, RNA8D* directed to HSPA8 pre-mRNA and RNA4502 directed to ribosomal RNAs. Control cells were incubated with Lipofectamine only. The level of mRNA was determined with quantitative RT-PCR and was represented as relative values normalized to the level of GAPDH mRNA. The error bars represent standard deviations. The asterisks (*) indicate significant difference from control .