Figure 5: (a) Time course of the HSPA8 mRNA RNA level in MCF-7 cells treated with artificial box C/D RNAs. RNA7 (160 nM) was transfected into cells using Lipofectamine, and then the cells were cultured for time intervals from 6 h to 72 h. Control cells (indicated as “0 h”) were incubated with Lipofectamine only. The error bars represent standard deviations. The asterisks (*) indicate significant difference from control . (b) Splicing variants of HSPA8 pre-mRNA after transfection of artificial box C/D RNA targeted to the branch point adenosine into MCF-7 cells. RNA7 (160 nM) was transfected into cells using Lipofectamine. Cells were cultured for the time indicated. Control cells were incubated with Lipofectamine only. The schematic structures of splicing forms are represented on the left, with II, III, IV, and V corresponding to the numbers of HSPA8 mRNA exons.