Figure 2: Effects of the knockdown of GalNAc4S-6ST on the pulmonary metastasis of LLC cells. Control- or GalNAc4S-6ST-shRNA/LLC-cell suspensions of 1 × 106 cells in 200 μL of DMEM were injected into a tail vein of C57BL/6J mice, and 21 days later, the number of tumor foci in the lungs was recorded. Six mice were used per group. Representative lungs from mice injected with the LLC cells stably expressing control- (mock) or GalNAc4S-6ST-shRNA (4S6ST-shRNA) are shown in (a). The number of colonies (b) and the wet weight (c) of the lungs from mice injected with each LLC clone were measured. Data represent the mean ± S.D. for two independent experiments. * versus control by one-way ANOVA with Dunnett’s adjustment.