Figure 5: Effects of the knockdown of GalNAc4S-6ST on the proliferation of LLC cells. LLC cells stably expressing control-shRNA (mock) or GalNAc4S-6ST-shRNA (4S6ST-shRNA) were seeded on 96-well plates at 2 × 103 cells/well and incubated at 37°C for the assessed period. Cell numbers were measured every 24 h using TetraColor One reagent containing a tetrazolium and electronic carrier as described in Section 2. The data represent the mean ± S.D. for three clones of both control-shRNA (nos. 5, 10, and 14) and GalNAc4S-6ST-shRNA (nos. 7, 17, and 23). Three independent experiments were performed, and representative results are shown. * versus control-shRNA by Student’s t-test.