Combined Treatment with Low Concentrations of Decitabine and SAHA Causes Cell Death in Leukemic Cell Lines but Not in Normal Peripheral Blood Lymphocytes
Figure 6
Apoptotic effects of one shot versus sequential DAC treatment. (a) Control cells (C) were treated by 1 μM DAC for 48 h (D) or sequentially treated by two doses of 0.5 μM DAC (DD1) or 1 μM DAC (DD2) every 24 h in the presence or absence of 1 μM SAHA. Treated cells were fixed in EtOH and stained by PI and the fraction of cells in subG1 phase of cell cycle was analyzed. Error bars represent ±SEM of four measurements. Statistical significance degree of difference: (*). (b) Immunoblots showing executive caspases activation in samples are described in (a).
