674378.fig.006a
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674378.fig.006b
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Figure 6: Composite photomicrographs of H&E stained longitudinal sections from the tibias cortex of control goat (4818) (a) and treated goat (4820) (b). 2X magnification photomicrographs (scale bars = 1 mm) show large periosteal callus (a,b—vertical double-headed arrows) composed of woven bone and fibrous connective tissue surrounding the osteotomy sites, which are not bridged by callus (a,b—horizontal double-headed arrows). Lysis of adjacent cortical bone (a,b—C) is evident, and inflammation (a,b—asterisks) concentrated at the osteotomy site dissects through the cortex of 4818. Folds in the cortex of 4820 (b, white arrows) are artifacts of processing. 20X magnification photomicrographs (insets, scale bars = 50 μm) show necrotic debris (a, inset—asterisk) surrounded by inflammation (a, inset—i) and microsequestra (a, inset—arrow). A large bacteria colony surrounded by radiating arrays of proteinaceous material (b, inset—asterisk), consistent with Splendore-Hoeppli formation is surrounded by inflammation (b, inset—i).