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BioMed Research International
Volume 2013 (2013), Article ID 675145, 8 pages
http://dx.doi.org/10.1155/2013/675145
Research Article

Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey

1Department of Parasitology, Faculty of Medicine, Cumhuriyet University, 58140 Sivas, Turkey
2Department of Biology, Faculty of Science and Letters, Kahramanmaraş Sütçü İmam University, 46100 Kahramanmaras, Turkey
3Department of Infectious Diseases, Faculty of Medicine, Cumhuriyet University, 58140 Sivas, Turkey
4Department of Biochemistry, Faculty of Pharmacology, Cumhuriyet University, 58140 Sivas, Turkey
5CUTFAM Research Center, Faculty of Medicine, Cumhuriyet University, 58140 Sivas, Turkey

Received 9 April 2013; Revised 11 June 2013; Accepted 27 June 2013

Academic Editor: Gernot Zissel

Copyright © 2013 Kübra Açıkalın Coşkun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present work focuses on a local survey of free-living amoebae (FLA) that cause opportunistic and nonopportunistic infections in humans. Determining the prevalence of FLA in water sources can shine a light on the need to prevent FLA related illnesses. A total of 150 samples of tap water were collected from six districts of Sivas province. The samples were filtered and seeded on nonnutrient agar containing Escherichia coli spread. Thirty-three (22%) out of 150 samples were found to be positive for FLA. The FLA were identified by morphology and by PCR using 18S rDNA gene. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of three different species, Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis. Naegleria fowleri, Balamuthia mandrillaris, or Sappinia sp. was not isolated during the study. All A. castellanii and A. polyphaga sequence types were found to be genotype T4 that contains most of the pathogenic Acanthamoeba strains. The results indicated the occurrence and distribution of FLA species in tap water in these localities of Sivas, Turkey. Furthermore, the presence of temperature tolerant Acanthamoeba genotype T4 in tap water in the region must be taken into account for health risks.