Table 1: Primers used in this study ( ).

SpeciesForward primerReverse primerReference

Common for FLACGCGGTAATTCCAGCTCCAATAGCCAGGTTAAGGTCTCGTTCGTTAACTsvetkova et al., 2004 [7]
Acanthamoeba spp. GGCCCAGATCGTTTACCGTGTCTCACAAGCTGCTAGGGAGTCASchroeder et al., 2001 [22]
N. fowleri CAAACACCGTTATGACAGGGCTGGTTTCCCTCACCTTACGSchild et al., 2007 [23]
B. mandrillaris CGCATGTATGAAGAAGACCATTACCTATATAATTGTCGATACCABooton et al., 2003 [24]
Sappinia TCT GGT CGC AAG GCT GAA ACGCA CCA CCA CCC TTG AAA TCQvarnstrom et al., 2009 [10]

FLA: Free-living amoebae.