P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

Figure 6

Method responses differential as a function of mean fluorescence. The graph shows the differences between fluorescence measured in flow cytometry (FL1-FC) and microplate reader (cell-normalized well fluorescence) as function of mean fluorescence measured in these two methods. Gap between flow cytometry and microplate reader responses linearly increased with P-gp blockers-induced fluorescence accumulation.