About this Journal Submit a Manuscript Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 676845, 11 pages
http://dx.doi.org/10.1155/2013/676845
Research Article

P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods

1Laboratory of Ecotoxicology UPRES EA 3222, IFRMP 23, University of Le Havre, 76058 Le Havre Cedex, France
2Department of Genetic Medicine, Weill Cornell Medical College, New York, NY 10022, USA
3Stem Cell and Microenvironment Laboratory, Weill Cornell Medical College in Qatar, Doha 24144, Qatar

Received 17 June 2013; Accepted 29 September 2013

Academic Editor: Beric Henderson

Copyright © 2013 Jennifer Pasquier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Pluen, Y. Boucher, S. Ramanujan et al., “Role of tumor-host interactions in interstitial diffusion of macromolecules: cranial versus subcutaneous tumors,” Proceedings of the National Academy of Sciences of the United States of America, vol. 98, no. 8, pp. 4628–4633, 2001. View at Publisher · View at Google Scholar · View at Scopus
  2. B. J. Druker, C. L. Sawyers, H. Kantarjian et al., “Activity of a specific inhibitor of the BCR-ABL tyrosine kinase in the blast crisis of chronic myeloid leukemia and acute lymphoblastic leukemia with the Philadelphia chromosome,” The New England Journal of Medicine, vol. 344, no. 14, pp. 1038–1042, 2001. View at Publisher · View at Google Scholar · View at Scopus
  3. J. L. Biedler and H. Riehm, “Cellular resistance to actinomycin D in Chinese hamster cells in vitro: cross-resistance, radioautographic, and cytogenetic studies,” Cancer Research, vol. 30, no. 4, pp. 1174–1184, 1970. View at Scopus
  4. J. A. Endicott and V. Ling, “The biochemistry of P-glycoprotein-mediated multidrug resistance,” Annual Review of Biochemistry, vol. 58, pp. 137–171, 1989. View at Publisher · View at Google Scholar · View at Scopus
  5. I. Bosch and J. Croop, “P-glycoprotein multidrug resistance and cancer,” Biochimica et Biophysica Acta, vol. 1288, no. 2, pp. F37–F54, 1996. View at Publisher · View at Google Scholar · View at Scopus
  6. S. V. Ambudkar, S. Dey, C. A. Hrycyna, M. Ramachandra, I. Pastan, and M. M. Gottesman, “Biochemical, cellular, and pharmacological aspects of the multidrug transporter,” Annual Review of Pharmacology and Toxicology, vol. 39, pp. 361–398, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. S. V. Ambudkar, C. Kimchi-Sarfaty, Z. E. Sauna, and M. M. Gottesman, “P-glycoprotein: from genomics to mechanism,” Oncogene, vol. 22, no. 47, pp. 7468–7485, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. A. H. Schinkel, “The physiological function of drug-transporting P-glycoproteins,” Seminars in Cancer Biology, vol. 8, no. 3, pp. 161–170, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. I. B. Holland and M. A. Blight, “ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans,” Journal of Molecular Biology, vol. 293, no. 2, pp. 381–399, 1999. View at Publisher · View at Google Scholar · View at Scopus
  10. D. Turk and G. Szakacs, “Relevance of multidrug resistance in the age of targeted therapy,” Current opinion in Drug Discovery & Development, vol. 12, pp. 246–252, 2009.
  11. B. J. Trock, F. Leonessa, and R. Clarke, “Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp170 expression and its possible functional significance,” Journal of the National Cancer Institute, vol. 89, no. 13, pp. 917–931, 1997. View at Publisher · View at Google Scholar · View at Scopus
  12. I. F. Faneyte, P. M. Kristel, and M. J. van de Vijver, “Determining MDR1/P-glycoprotein expression in breast cancer,” The International Journal of Cancer, vol. 93, pp. 114–122, 2001.
  13. W. T. Beck, T. M. Grogan, C. L. Willman et al., “Methods to detect P-glycoprotein-associated multidrug resistance in patients' tumors: consensus recommendations,” Cancer Research, vol. 56, no. 13, pp. 3010–3020, 1996. View at Scopus
  14. H. J. Broxterman, P. Sonneveld, N. Feller et al., “Quality control of multidrug resistance assays in adult acute leukemia: correlation between assays for P-glycoprotein expression and activity,” Blood, vol. 87, no. 11, pp. 4809–4816, 1996. View at Scopus
  15. J. Meesungnoen, J.-P. Jay-Gerin, and S. Mankhetkorn, “Relation between MDR1 mRNA levels, resistance factor, and the efficiency of P-glycoprotein-mediated efflux of pirarubicin in multidrug-resistant K562 sublines,” Canadian Journal of Physiology and Pharmacology, vol. 80, no. 11, pp. 1054–1063, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. J. Pasquier, L. Galas, C. Boulangé-Lecomte et al., “Different modalities of intercellular membrane exchanges mediate cell-to-cell P-glycoprotein transfers in MCF-7 breast cancer cells,” The Journal of Biological Chemistry, vol. 287, no. 10, pp. 7374–7387, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. O. Legrand, G. Simonin, J. Y. Perrot, R. Zittoun, and J. P. Marie, “Pgp and MRP activities using calcein-AM are prognostic factors in adult acute myeloid leukemia patients,” Blood, vol. 91, no. 12, pp. 4480–4488, 1998. View at Scopus
  18. L. Homolya, Z. Hollo, U. A. Germann, I. Pastan, M. M. Gottesman, and B. Sarkadi, “Fluorescent cellular indicators are extruded by the multidrug resistance protein,” The Journal of Biological Chemistry, vol. 268, no. 29, pp. 21493–21496, 1993. View at Scopus
  19. M. Marin, H. Legros, A. Poret, F. Leboulenger, and F. Le Foll, “Cell responses to xenobiotics: comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms,” Marine Environmental Research, vol. 58, no. 2–5, pp. 209–213, 2004. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Pasquier, P. Magal, C. Boulangé-Lecomte, G. Webb, and F. Le Foll, “Consequences of cell-to-cell P-glycoprotein transfer on acquired multidrug resistance in breast cancer: a cell population dynamics model,” Biology Direct, vol. 6, article 5, 2011. View at Publisher · View at Google Scholar · View at Scopus
  21. D. G. Altman and J. M. Bland, “Comparison of methods of measuring blood pressure,” Journal of Epidemiology and Community Health, vol. 40, no. 3, pp. 274–277, 1986. View at Scopus
  22. C. Maulard, J.-P. Marie, S. Delanian, and M. Housset, “Clinical effects on CA 15-3 level of cyclosporin A as a chemosensitiser in chemoresistant metastatic breast cancer,” European Journal of Cancer A, vol. 29, no. 3, p. 480, 1993. View at Publisher · View at Google Scholar · View at Scopus