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Figure 2: Comparison between the average morphometric parameters of the myelinated fibers and respective axons from the sural nerves of young (90-day-old) female Wistar rats, obtained by the automatic and semiautomated methods. Data presented as average ± standard error of mean. * indicates significant difference compared to automatic method (nonparametric test of Mann-Whitney for ratio values and unpaired Student’s -test for fiber, axon and myelin sheath area, and fiber and axon diameter).