Table 1: Average morphometric parameters of the myelinated fibers from the proximal segments of the sural nerve of young (90-day-old) female Wistar rats, obtained by three different observers, using the semiautomated method.

Observer 1Observer 2Observer 3

MF area (µm2)
MF diameter (µm)
AX area (µm2)
AX diameter (µm)
Myelin area (µm2)
G ratio
MF counted
MF measured

MF: myelinated fiber; AX: myelinated axon. *indicates significant difference compared to observer 1; #indicates significant difference compared to observer 2; indicates significant difference compared to observer 3 (one-way analysis of variance followed by the post hoc test of Holm-Sidak).