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BioMed Research International
Volume 2013 (2013), Article ID 683405, 10 pages
The Immunobiology of Prostanoid Receptor Signaling in Connecting Innate and Adaptive Immunity
Faculty of Dental Medicine, University of Monastir, 5019 Monastir, Tunisia
Received 30 April 2013; Revised 8 July 2013; Accepted 21 July 2013
Academic Editor: Sherven Sharma
Copyright © 2013 Hedi Harizi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Prostanoids, including prostaglandins (PGs), thromboxanes (TXs), and prostacyclins, are synthesized from arachidonic acid (AA) by the action of Cyclooxygenase (COX) enzymes. They are bioactive inflammatory lipid mediators that play a key role in immunity and immunopathology. Prostanoids exert their effects on immune and inflammatory cells by binding to membrane receptors that are widely expressed throughout the immune system and act at multiple levels in innate and adaptive immunity. The immunoregulatory role of prostanoids results from their ability to regulate cell-cell interaction, antigen presentation, cytokine production, cytokine receptor expression, differentiation, survival, apoptosis, cell-surface molecule levels, and cell migration in both autocrine and paracrine manners. By acting on immune cells of both systems, prostanoids and their receptors have great impact on immune regulation and play a pivotal role in connecting innate and adaptive immunity. This paper focuses on the immunobiology of prostanoid receptor signaling because of their potential clinical relevance for various disorders including inflammation, autoimmunity, and tumorigenesis. We mainly discuss the effects of major COX metabolites, PGD2, PGE2, their signaling during dendritic cell (DC)-natural killer (NK) reciprocal crosstalk, DC-T cell interaction, and subsequent consequences on determining crucial aspects of innate and adaptive immunity in normal and pathological settings.
Prostanoids are biologically active molecules that have various and potent effects on almost all cells and tissues in physiological and pathophysiological conditions . These fascinating molecules can sustain homeostatic functions and mediate pathogenic mechanisms, including tumorigenesis autoimmunity, cardiovascular diseases, and inflammation . Known as potent inflammatory lipid mediators, prostanoids may function in both the promotion and resolution of the inflammatory response . Their biosynthesis is suppressed by nonsteroidal anti-inflammatory drugs (NSAIDs) that are clinically relevant molecules widely consumed as analgesics or antipyretics drugs. The use of NSAIDs as anti-inflammatory agents highlights the proinflammatory role of prostanoids. For example, NSAIDs reduce fever caused by infectious, inflammatory, or neoplastic diseases, by inhibiting the synthesis of PGE2 . Moreover, epidemiological studies have provided evidence that NSAIDs that inhibit PG synthesis by acting on COX enzymes can significantly reduce the risk of cancer development , suggesting that prostanoids may play a key role in tumorigenesis. In addition, defect in antitumor immunity has been reported to be associated with increased expression of COX-2-derived PGE2 [6####^~^~^~^~^~^####x2013;8].
Cell activation by growth factors, inflammatory stimuli, or mechanical trauma, resulted in the induction of prostanoid synthesis . Rapid recognition of microbial lipopolysaccharide (LPS) by toll-like receptor-4 (TLR-4) is an important pathway, which provides an ideal model for the activation of prostanoid production and signaling . Several lines of evidence showed that LPS of gram-negative bacteria induces the expression and activity of cytosolic phospholipase A2 (cPLA2), which catalyzes the release of endogenous AA from the cell membrane [11, 12]. The expression and activity of cPLA2 have been documented in various cell types, such as human leukocytes and murine dendritic cells (DCs) [13####^~^~^~^~^~^####x2013;15]. AA released from cell membrane by the action of cPLA2 can be converted by COX enzymes into the unstable cyclic endoperoxides PGG2 and PGH2. Specific downstream isomerases and synthases are involved in the biosynthesis of many prostanoids, including TXs, PGs, and prostacyclins .
COX enzymes exist as two distinct isoforms, COX-1 and COX-2. COX-1 is a housekeeping gene expressed constitutively in most cells and involved in physiological processes, such as gastric epithelial cytoprotection and homeostasis. COX-2 is usually absent under basal conditions but can be induced by several stimuli, such as cytokines and other inflammatory factors. COX-2 enzyme can be induced by bacterial LPS mimicking bacterial infection . Physiological activation of CD40-CD40 Ligand pathway, which occurs during antigen presentation by DCs, can induce the expression of COX-2 enzyme and the production of proinflammatory PGs . CD40-induced production of some prostanoids has also been found in other cell types, such as fibroblasts , endothelial cells , and monocytes .
COX-2 pathway is an important source of prostanoid formation in inflamed tissue and cancer [22, 23]. There is emerging evidence that COX-2-derived prostanoids, mainly PGD2 and PGE2, play a crucial role in the function of all the components of the immune system . In addition, the crosstalk between immune cells that influences subsequent adaptive immune responses can be modulated by PGD2 and PGE2 receptor signaling. Given the potent immunomodulatory effects of PGD2 and PGE2 [25####^~^~^~^~^~^####x2013;27], it is not surprising that cells that produced large amounts of these lipid mediators are considered to be the most powerful modulators of inflammatory processes and immune function. For these reasons, understanding how prostanoid receptor signaling affect immune cell crosstalk and functions may be exploited for rational development of immunotherapeutic strategies in various diseases ranging from inflammation and allergic diseases to cancer. Investigating the role of prostanoids in connecting innate and adaptive immunity is very complex because of differential profile of prostanoid biosynthesis within immune cells, multiple prostanoid-synthesizing enzymes, and different receptor signaling pathways with sometimes opposite effects. Moreover, epigenetic modifications in prostanoid genes represent additional levels of complexity for understanding immunological processes involved in normal and pathological settings.
2. Differential Profile of Prostanoid Biosynthesis in Immune Cells
Depending on the prostanoid type, activated signaling pathway, and cellular target, prostanoids exert different and sometimes opposite actions . These lipid mediators are distributed in all tissues and the profile of prostanoid production depends on the cell type and the differential expression and distribution of enzymes involved in their biosynthetic pathways within cells involved in generating inflammatory response. For example, mast cells that are resident immune cells, mainly located in the lung, gut, and skin, are known to be major producer of PGD2 when activated in response to allergen exposure [29, 30]. Other cell types, such as platelets, alveolar macrophages, Th2 cells, and osteoblasts, can also express PGD2-synthesizing enzymes and produce PGD2, albeit at much lower levels .
Among immune and inflammatory cells, macrophages have been reported to be an important source of AA-derived metabolites . They are able to produce various lipid mediators, especially TXA2 and PGE2, the most abundant PGs produced in the body . The profile of prostanoid production by macrophages can be altered upon cellular activation. While resting macrophages produce TXA2 in excess of PGE2, this ratio changes to favor PGE2 production in response to LPS activation . Other cell types, such as fibroblasts, endothelial cells, and some types of malignant cells can produce PGE2. Human monocyte-derived DCs and bone marrow-derived DCs, the most potent antigen presenting cells (APC) of the immune system, produced high levels of PGE2, but not PGD2, as we and others have previously reported [34####^~^~^~^~^~^####x2013;36]. In another study, it has been reported that skin DCs express hematopoietic PGD synthase and also function as a source of PGD2, which can be nonenzymatically converted to different physiological metabolites, particularly 15-deoxy-####^~^~^~^~^~^####x394;12,14 prostaglandin J2 (15d-PGJ2), known as a potent anti-inflammatory factor [37, 38].
The general consensus is that cells of the innate immunity, such as tissue macrophages and sentinel DCs, are major contributors of local prostanoids . However, cells of adaptive immune response have been reported to be unable to synthesize prostanoids. The only exception is adaptive regulatory T cells as they express COX-2 enzyme and synthesize high levels of PGE2 . Generated by immune or nonimmune cells, prostanoids can act locally in an autocrine and paracrine fashion and affect the function of neighboring cells. It has been reported that the endogenously released prostanoids represent an important mechanism by which APCs, including DCs and macrophages, regulate their own function [41, 42] and the function of other cell types, such as T, B, and NK cells [43, 44]. Although some cells of innate and adaptive immunity appeared to be unable to synthesize prostanoids, such as PGE2 and PGD2, they are described as powerful prostanoid-responding cells, as they express various prostanoid receptors.
3. Prostanoid Receptor Expression and Signaling
Studies on AA-derived lipid mediators showed that cells of the immune system are both sensitive to and a source of inflammatory prostanoids . These lipid mediators exert their autocrine and/or paracrine functions through various receptor types with different location and signaling pathways . Given the evanescent nature of some prostanoids, especially thromboxane and prostacyclin that have half-lives on the order of seconds to a few minutes, these compounds must act near their sites of synthesis.
The major effects of prostanoid lipid mediators are mediated through specific G-protein-coupled cell membrane receptors that differ in their expression and intracellular signaling pathways . PGE2, one of the best known and most well-studied prostanoids, exerts its effects through four distinct receptor subtypes (EP1####^~^~^~^~^~^####x2013;EP4). Two PGD2 receptors termed DP1 and DP2 bind PGD2 and mediate its effects on target cells [47, 48]. The receptors that bind PGF2####^~^~^~^~^~^####x3b1;, PGI2, and TxA2 are FP, IP, and TP, respectively. EP2, EP4, IP, and DP1 signal through Gs-mediated increases in intracellular cyclic adenosine monophosphate (cAMP). However, EP1, FP, and TP activate phosphatidylinositol metabolism, leading to the formation of inositol trisphosphate with mobilization of intracellular Ca2+ stores. The PGD2 receptor DP2 also termed CRTH2 (chemoattractant receptor-homologous molecule) is expressed on Th2 lymphocytes  and is coupled with -type G protein .
Although the major effects of COX-derived lipid mediators are mediated through cell surface receptors, some prostanoids and their metabolites may act on target cells through nuclear receptors located at the nuclear envelope . The most important example is 15d-PGJ2, the physiological bioactive metabolite of PGD2, and the natural ligand for the Peroxisome proliferator-activated receptor-####^~^~^~^~^~^####x3b3; (PPAR####^~^~^~^~^~^####x3b3;) , which is a member of the nuclear receptor superfamily of ligand-dependent transcription factors. 15d-PGJ2 has garnered much interest because despite of its role in cytoprotection and inhibition of cellular proliferation , it possesses potent anti-inflammatory properties by inhibiting the transcription of pro-inflammatory mRNAs .
It has been reported that cellular components of the immune system express various prostanoid receptors . The distribution of prostanoid receptors on immune cells differs from the distribution of prostanoid-specific synthases. While prostanoid receptors are expressed on cells of both innate (APC) and adaptive (T and B lymphocytes) immune systems, prostanoid synthases, on the other hand, are expressed mainly by the cells of inflammation (phagocytes) but not on T and B lymphocytes. Thus, stimulated cells of innate immunity, at a site of inflammation, will produce prostanoids, which in turn, will modulate their function in an autocrine fashion. Prostanoids will also contribute, with other inflammatory mediators, to the regulation of adaptive immunity through the innate system. These data clearly demonstrated that by their paracrine signaling, prostanoid receptors play critical roles in connecting innate and adaptive immune responses [16, 28]. The paracrine action of the endogenously produced prostanoids has been clearly documented in some immune organs and tissues. For example, cells of the thymus microenvironment, including those from the monocyte-macrophage lineage produce prostanoids, which contribute to the education of immature thymocytes. They also promote or block tolerance to self- and nonself-antigens. For example, tolerance to self might result from PGE2-driven apoptosis .
4. Prostanoid Receptor Signaling at the Interface between Innate and Adaptive Immunity
Receptors for prostanoids emerged as key regulators for both innate and adaptive immune responses, since they are widely expressed throughout the immune system, and function at multiple levels in connecting the innate and adaptive immunity [16, 24]. Biologically active prostanoids have a great impact on the phenotype and function of immune cells [27, 55]. Many accumulating data have reported that the production of inflammatory mediators and the expression of their receptors during the interaction of various immune cells markedly affect the outcome of immune responses in health and diseases [24, 33, 56]. Recognized as major prostanoids produced by immune cells, PGD2 and PGE2 have garnered much interest because they act as potent regulators of cell-cell interaction, antigen presentation, cytokine production, cytokine receptor expression, differentiation, survival, apoptosis, cell-surface molecule levels, and cell migration in both autocrine and paracrine manners. PGE2 can profoundly modulate various aspects of the immune and inflammatory responses [27, 33]. PGD2 has long been considered as a crucial regulator of allergic responses [57, 58]. However, it might exert many immunologically relevant anti-inflammatory functions in several experimental models [59####^~^~^~^~^~^####x2013;61]. In addition, it has been reported that mast cell-derived PGD2 acts as an antiangiogenic factor in expanding lung carcinoma . Here, we focused on the immunobiology of PGD2 and PGE2 receptor signaling in tow examples of immune cell interactions: the first is the bidirectional activating crosstalk between DCs and NK cells and its crucial role in innate and adaptive immune regulations, the second is the physiological dialog between DCs and T cells during antigen presentation and the generation of adaptive immune responses in normal and pathological settings.
4.1. PGE2 and PGD2 Signaling during DC-NK Cell Crosstalk
Although various cell types participate in innate and adaptive immune regulations, DCs are a rather unique cell type, in that they may function in both innate and adaptive immunity, depending on their state of maturation and local microenvironmental conditions . After their development from hematopoietic stem cells (HSCs) in the bone marrow, immature DCs migrate to the periphery and reside in nonlymphoid organs where they actively take up the antigens from the extracellular fluid . In response to signal of inflammatory microenvironment, immature DCs undergo a process of maturation triggered by a variety of pathogen related molecules, such as bacterial LPS. They subsequently leave the tissues via the afferent lymphatic vessels, enter the draining lymph nodes in the T-cell-rich zone, present the processed antigens to naive T cells and induce a specific primary immune response . DC maturation and migration involve soluble mediators, such as cytokines and prostanoids .
In addition to their originally and historically known function as the only professional APC capable of activating na####^~^~^~^~^~^####xef;ve T cells, DCs are also characterized with their potent ability to interact with innate immune cells, especially NK cells . In fact, DCs and NK cells can interact with each other, and depending on the activation status of both cell types, this reciprocal dialog may lead to NK cell activation, DC activation, or apoptosis . By modulating NK cell proliferation, IFN####^~^~^~^~^~^####x3b3; production , and cytotoxic activity , DCs play a pivotal role in orchestrating NK cell-mediated innate immune responses . Conversely, NK cells can regulate the maturation, cytokine production, and immunostimulatory capacity of DCs . The interaction between DCs and NK cells can be mediated through cell-cell contact, membrane-bound ligands, or by a variety of soluble factors, such cytokines and prostanoids that contribute to the modulation of the activity of both cell types. The reciprocal activating crosstalk between DCs and NK cells appeared to be markedly affected by PGE2 in normal and pathological settings . Innate and effector functions of NK cells that require close interactions with activated DCs can be modulated by PGE2. In fact, NK cell functions (lysis, migration, proliferation, and cytokine production) are markedly influenced by DC-derived PGE2, which acts as a potent suppressor of DC-NK cell crosstalk (Figure 1(a)). During DC-NK cell crosstalk, DCs produce PGE2, which binds EP receptors expressed at the surface of NK cells  and suppresses their functions. Although NK cells express all PGE2 receptors , EP2 and/or EP4 receptor signaling appeared to play the main role in this effect [71, 72]. The suppression of DC-NK cell crosstalk by PGE2 can be mediated through the modulation of DC released chemokines and cytokines that are involved in NK cell recruitment [34, 73]. In some cases, defects in DC function resulted in the release of immunosuppressive factors, such PGE2 [6, 74, 75], which can induce the production of IL-10. This cytokine acts as a potent suppressor of the bidirectional activating crosstalk between NK cells and DCs . Moreover, overproduction of immunosuppressive PGE2 has been clearly established in many cancers , causing reduced number of tumor-infiltrating DCs and a reduction in their APC function . Defect in DC differentiation and APC function and cancer-associated immunodeficiency have been reported to be mediated by PGE2 EP2 receptor signaling .
Accumulating data reported that PGE2 EP2 and EP4 receptor signaling are potent immunoregulatory pathways affecting maturation, migration cytokine production, and Th cell-polarizing ability of DCs in health and diseases [70, 78, 79]. The general consensus is that EP1 and EP3 receptors have no role in DC biology as we and others have previously demonstrated [42, 78]. However, the recently published data by Singh et al.  showed that PGE2 affects Flt3L-dependent DC development from hematopoietic progenitor cells through EP1 and EP3 receptor-dependent mechanism, and DC generation was markedly lower in EP1 and/or EP3 knockout mice.
Although the most intensively studied PG member is PGE2, PGD2 emerged as a very interesting COX-2-derived metabolite involved in many biological processes [81, 82]. Described as a potent mediator with a key role in the development and pathogenesis of allergic diseases [83, 84], PGD2 can act as a potent regulator of immune and inflammatory responses [24, 27, 85, 86]. Within the immune system, PGD2 is generally recognized by its suppressive effects of cellular functions through DP1 receptor signaling, which is a powerful activator of the adenylate cyclase system and elevated levels of cAMP (Figure 1(b)). However, the stimulatory effects of PGD2 on type 2 cells are mediated by CRTH2 signal [47, 48]. Local production of PGD2 has been associated with impaired functions of NK cells in type 2 immune-mediated pathologies, such as asthma . In fact, NK cell cytotoxic activity, chemotaxis, and type 1 cytokine production have been reported to be suppressed by PGD2 through DP1 receptor-dependent mechanism .
During the inflammatory response, PGD2 appears to exert a dual function with the capacity to act as either a mediator or a potent inhibitor of inflammation in some physiological or pathological conditions [59, 60]. This lipid mediator greatly affects DC migratory capacity and markedly downregulates the production of the Th1-promoting cytokine IL-12 through DP1, but not DP2 receptor signaling . In superficial organs, such as skin and mucosa where DCs and mast cells are colocalized, PGD2 produced by mast cells can also suppress IL-12 release by DCs leading to Th2 polarized immune responses in vivo .
4.2. PGE2 and PGD2 Signaling during DC-T Cell Interaction
Activation of na####^~^~^~^~^~^####xef;ve T cells and their polarization into effector cells requires close interactions with mature DCs in the lymphoid organs. During the maturation process and/or during DC/T lymphocyte contact, many factors including cytokines and inflammatory lipid mediators, such as PGE2 and PGD2, can be produced (Figure 2(a)). Cytokine production and antigen presentation by DCs are inhibited by PGE2  through EP2 and/or EP4 receptors signaling [42, 90]. PGE2 is also known to induce many immunosuppressive factors, such as indoleamine 2, 3-dioxygenase (IDO) [91, 92], which can suppress T-cell proliferation and survival and induces immunological tolerance . The immunomodulatory effects of PGE2 can be prolonged and sustained by these immunosuppressive factors known by their ability to induce a tolerogenic type of DCs [15, 91]. Moreover, it has been reported that DCs, exposed to autocrine and/or paracrine PGE2, induce the differentiation of naive T cells into Th2 cells, which produce high levels of IL-4 and no IFN-####^~^~^~^~^~^####x3b3; .
Another COX metabolite that can be released during DC-T cell interaction is PGD2 (Figure 2(b)). In the lymphoid organs, the local production of PGD2 by DCs and/or T cells [95, 96] may affect the immune response by targeting Th1-driving cytokine IL-12 , suggesting a key role of PGD2 in the outcome of the adaptive immunity. PGD2 can greatly affect the migratory properties of DCs. Gosset et al.  reported that PGD2 and its metabolite 15d-PGJ2 are able to block the maturation of human monocyte-derived DCs (MD-DCs) in a DP receptor-dependent manner. Paracrine effects of PGD2 on DCs and T cells have also been shown . In fact, some immune cells, such as macrophages, might secrete PGD2, which suppresses DC migration and T cell activation. By blocking DC migration, PGD2 prevents further immune stimulation, thus contributing to the resolution of inflammation.
5. Epigenetic Modifications of Prostanoids: Fine Regulations with More Complexity
The potential relevance of prostanoid signaling in inflammation, cancer, or disease susceptibility and individual variations in drug responses will be an important area for investigation. The study of prostanoid involvement in connecting innate and adaptive immunity is a complex process. In addition to the environmental factors and the genetic background to diseases, including cancer and asthma, epigenetic mechanisms involved in the fine regulation of prostanoid biosynthesis and receptor signaling appeared to be crucial in controlling the different components of the COX pathways [98, 99]. In fact, genes encoding for inflammatory prostanoids and their receptors are subjected to epigenetic modifications by acetylation of core histone. Immune disorders, such as asthma and cancer, are characterized by the expression of various inflammatory genes that can be epigenetically regulated by acetylation of core histone. For example, increased activity of histone acetyltransferase and reduced activity of histone deacetylase have been observed in asthmatic patients . Histone acetylation was also found to be a critical regulator of EP expression in cancer . The epigenetic downregulation of the EP2 gene by DNA CpG methylation was observed to be associated with progression of neuroblastomas . Together, these data suggest that prostanoids and their receptors can be functionally regulated epigenetically, and the epigenetic mechanisms controlling the different components of prostanoid biosynthesis pathway and signaling should be considered in the development of therapeutic approaches aimed at targeting prostanoid biosynthesis and signaling in immune disorders.
6. Concluding Remarks
The molecular and cellular basis of the immune regulation by prostanoids in physiological and pathological situations remains a topic of great interest. Receptors for major prostanoids, especially PGD2 and PGE2, are widely expressed throughout the immune system, and function at multiple levels in connecting the innate and adaptive immunity. PGD2 and PGE2 receptor signaling emerged as key regulators for both innate and adaptive immune response. Both DC-T cell interactions and DC-NK cell reciprocal activating crosstalk provide a target for pharmacological interventions in normal and pathological settings. The manipulation of the local cellular prostanoids and their receptors expressed by innate and adaptive immune cells during their functional crosstalk might be an interesting approach to modulate DC and/or NK cell functions for specific immune responses, especially in cancer, asthma and inflammation.
This work was supported by Regional League against Cancer, Departmental Committee of Gironde and Charentes.
- H. Harizi, J.-B. Corcuff, and N. Gualde, “Arachidonic-acid-derived eicosanoids: roles in biology and immunopathology,” Trends in Molecular Medicine, vol. 14, no. 10, pp. 461–469, 2008.
- E. M. Smyth, T. Grosser, M. Wang, Y. Yu, and G. A. FitzGerald, “Prostanoids in health and disease,” Journal of lipid research, vol. 50, pp. S423–S428, 2009.
- E. Ricciotti and G. A. Fitzgerald, “Prostaglandins and inflammation,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 31, no. 5, pp. 986–1000, 2011.
- K. Walentynowicz, M. Szefer, B. Wojtal, P. Terlecki, S. Wrotek, and W. Kozak, “Role of prostaglandins in heme-induced fever,” Journal of Physiology and Pharmacology, vol. 57, no. 8, pp. 73–82, 2006.
- L. M. Coussens and Z. Werb, “Inflammation and cancer,” Nature, vol. 420, no. 6917, pp. 860–867, 2002.
- C. Ménétrier-Caux, C. Bain, M. C. Favrot, A. Duc, and J. Y. Blay, “Renal cell carcinoma induces interleukin 10 and prostaglandin E2 production by monocytes,” British Journal of Cancer, vol. 79, no. 1, pp. 119–130, 1999.
- G. P. Smyth, P. P. Stapleton, C. B. Barden et al., “Renal cell carcinoma induces prostaglandin E2 and T-helper type 2 cytokine production in peripheral blood mononuclear cells,” Annals of Surgical Oncology, vol. 10, no. 4, pp. 455–462, 2003.
- M. Ahmadi, D. C. Emery, and D. J. Morgan, “Prevention of both direct and cross-priming of antitumor CD8+ T-cell responses following overproduction of prostaglandin E2 by tumor cells in vivo,” Cancer Research, vol. 68, no. 18, pp. 7520–7529, 2008.
- C. D. Funk, “Prostaglandins and leukotrienes: advances in eicosanoid biology,” Science, vol. 294, no. 5548, pp. 1871–1875, 2001.
- E. Kuroda, T. Sugiura, K. Zeki, Y. Yoshida, and U. Yamashita, “Sensitivity difference to the suppressive effect of prostaglandin E2 among mouse strains: a possible mechanism to polarize Th2 type response in BALB/c mice,” Journal of Immunology, vol. 164, no. 5, pp. 2386–2395, 2000.
- A. A. Aderem, D. S. Cohen, S. D. Wright, and Z. A. Cohn, “Bacterial lipopolysaccharides prime macrophages for enhanced release of arachidonic acid metabolites,” Journal of Experimental Medicine, vol. 164, no. 1, pp. 165–179, 1986.
- K. Seibert, Y. Zhang, K. Leahy et al., “Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 25, pp. 12013–12017, 1994.
- E. Rodewald, U. Tibes, G. Maass, and W. Scheuer, “Induction of cytosolic phospholipase A2 in human leukocytes by lipopolysaccharide,” European Journal of Biochemistry, vol. 223, no. 3, pp. 743–749, 1994.
- M. Zaitsu, Y. Hamasaki, M. Matsuo et al., “Induction of cytosolic phospholipase A2 and prostaglandin H2 synthase-2 by lipopolysaccharide in human polymorphonuclear leukocytes,” European Journal of Haematology, vol. 63, no. 2, pp. 94–102, 1999.
- H. Harizi, M. Juzan, V. Pitard, J.-F. Moreau, and N. Gualde, “Cyclooxygenase-2-issued prostaglandin E2 enhances the production of endogenous IL-10, which down-regulates dendritic cell functions,” Journal of Immunology, vol. 168, no. 5, pp. 2255–2263, 2002.
- H. Harizi and N. Gualde, “The impact of eicosanoids on the crosstalk between innate and adaptive immunity: the key roles of dendritic cells,” Tissue Antigens, vol. 65, no. 6, pp. 507–514, 2005.
- H. Harizi, M. Juzan, C. Grosset, M. Rashedi, and N. Gualde, “Dendritic cells issued in vitro from bone marrow produce PGE2 that contributes to the immunomodulation induced by antigen-presenting cells,” Cellular Immunology, vol. 209, no. 1, pp. 19–28, 2001.
- H. Harizi, I. Limem, and N. Gualde, “CD40 engagement on dendritic cells induces cyclooxygenase-2 and EP2 receptor via p38 and ERK MAPKs,” Immunology and Cell Biology, vol. 89, no. 2, pp. 275–282, 2011.
- Y. Zhang, H. J. Cao, B. Graf, H. Meekins, T. J. Smith, and R. P. Phipps, “Cutting edge: CD40 engagement up-regulates cyclooxygenase-2 expression and prostaglandin E2 production in human lung fibroblasts,” Journal of Immunology, vol. 160, no. 3, pp. 1053–1057, 1998.
- A. I. Dongari-Bagtzoglou, U. Thienel, and M. J. Yellin, “CD40 ligation triggers COX-2 expression in endothelial cells: evidence that CD40-mediated IL-6 synthesis is COX-2-dependent,” Inflammation Research, vol. 52, no. 1, pp. 18–25, 2003.
- Y. Inoue, T. Otsuka, H. Niiro et al., “Novel regulatory mechanisms of CD40-induced prostanoid synthesis by IL-4 and IL-10 in human monocytes,” Journal of Immunology, vol. 172, no. 4, pp. 2147–2154, 2004.
- T. Hida, Y. Yatabe, H. Achiwa et al., “Increased expression of cyclooxygenase 2 occurs frequently in human lung cancers, specifically in adenocarcinomas,” Cancer Research, vol. 58, no. 17, pp. 3761–3764, 1998.
- S. Gately, “The contributions of cyclooxygenase-2 to tumor angiogenesis,” Cancer and Metastasis Reviews, vol. 19, no. 1-2, pp. 19–27, 2000.
- S. G. Harris, J. Padilla, L. Koumas, D. Ray, and R. P. Phipps, “Prostaglandins as modulators of immunity,” Trends in Immunology, vol. 23, no. 3, pp. 144–150, 2002.
- D. F. Legler, M. Bruckner, E. Uetz-von Allmen, and P. Krause, “Prostaglandin E2 at new glance: novel insights in functional diversity offer therapeutic chances,” International Journal of Biochemistry and Cell Biology, vol. 42, no. 2, pp. 198–201, 2010.
- I. R. Jowsey, A. M. Thomson, J. U. Flanagan et al., “Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases,” Biochemical Journal, vol. 359, no. 3, pp. 507–516, 2001.
- C. Faveeuw, P. Gosset, F. Bureau et al., “Prostaglandin D2 inhibits the production of interleukin-12 in murine dendritic cells through multiple signaling pathways,” European Journal of Immunology, vol. 33, no. 4, pp. 889–898, 2003.
- T. Hirata and S. Narumiya, “Prostanoids as regulators of innate and adaptive immunity,” Advances in Immunology, vol. 116, pp. 143–174, 2012.
- A. N. Hata and R. M. Breyer, “Pharmacology and signaling of prostaglandin receptors: multiple roles in inflammation and immune modulation,” Pharmacology and Therapeutics, vol. 103, no. 2, pp. 147–166, 2004.
- M. A. Gallant, R. Samadfam, J. A. Hackett, J. Antoniou, J.-L. Parent, and A. J. De Brum-Fernandes, “Production of prostaglandin D2 by human osteoblasts and modulation of osteoprotegerin, RANKL, and cellular migration by DP and CRTH2 receptors,” Journal of Bone and Mineral Research, vol. 20, no. 4, pp. 672–681, 2005.
- A. D. Luster and A. M. Tager, “T-cell trafficking in asthma: lipid mediators grease the way,” Nature Reviews Immunology, vol. 4, no. 9, pp. 711–724, 2004.
- S. W. Chensue and S. L. Kunkel, “Arachidonic acid metabolism and macrophage activation,” Clinics in Laboratory Medicine, vol. 3, no. 4, pp. 677–694, 1983.
- S. L. Tilley, T. M. Coffman, and B. H. Koller, “Mixed messages: modulation of inflammation and immune responses by prostaglandins and thromboxanes,” Journal of Clinical Investigation, vol. 108, no. 1, pp. 15–23, 2001.
- N. Gualde and H. Harizi, “Prostanoids and their receptors that modulate dendritic cell-mediated immunity,” Immunology and Cell Biology, vol. 82, no. 4, pp. 353–360, 2004.
- D. S. Whittaker, K. S. Bahjat, L. L. Moldawer, and M. J. Clare-Salzler, “Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production,” Journal of Immunology, vol. 165, no. 8, pp. 4298–4304, 2000.
- M. Fogel-Petrovic, J. A. Long, D. A. Knight, P. J. Thompson, and J. W. Upham, “Activated human dendritic cells express inducible cyclo-oxygenase and synthesize prostaglandin E2 but not prostaglandin D2,” Immunology and Cell Biology, vol. 82, no. 1, pp. 47–54, 2004.
- C. Shimura, T. Satoh, K. Igawa et al., “Dendritic cells express hematopoietic prostaglandin D synthase and function as a source of prostaglandin D2 in the skin,” American Journal of Pathology, vol. 176, no. 1, pp. 227–237, 2010.
- T. Shibata, M. Kondo, T. Osawa, N. Shibata, M. Kobayashi, and K. Uchida, “15-Deoxy--prostaglandin J2. A prostaglandin D2 metabolite generated during inflammatory processes,” Journal of Biological Chemistry, vol. 277, no. 12, pp. 10459–10466, 2002.
- R. P. Phipps, S. H. Stein, and R. L. Roper, “A new view of prostaglandin E regulation of the immune response,” Immunology Today, vol. 12, no. 10, pp. 349–352, 1991.
- M. Mahic, S. Yaqub, C. C. Johansson, K. Taskén, and E. M. Aandahl, “FOXP3+CD4+CD25+ adaptive regulatory T cells express cyclooxygenase-2 and suppress effector T cells by a prostaglandin E2-dependent mechanism,” Journal of Immunology, vol. 177, no. 1, pp. 246–254, 2006.
- S. W. Russell and J. L. Pace, “Both the kind and magnitude of stimulus are important in overcoming the negative regulation of macrophage activation by PGE2,” Journal of Leukocyte Biology, vol. 35, no. 3, pp. 291–301, 1984.
- H. Harizi, C. Grosset, and N. Gualde, “Prostaglandin E2 modulates dendritic cell function via EP2 and EP4 receptor subtypes,” Journal of Leukocyte Biology, vol. 73, no. 6, pp. 756–763, 2003.
- E. R. Fedyk and R. P. Phipps, “Prostaglandin E2 receptors of the EP2 and EP4 subtypes regulate activation and differentiation of mouse B lymphocytes to IgE-secreting cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 20, pp. 10978–10983, 1996.
- B. Rocca, L. M. Spain, E. Puré, R. Langenbach, C. Patrono, and G. A. FitzGerald, “Distinct roles of prostaglandin H synthases 1 and 2 in T-cell development,” Journal of Clinical Investigation, vol. 103, no. 10, pp. 1469–1477, 1999.
- S. Narumiya and G. A. FitzGerald, “Genetic and pharmacological analysis of prostanoid receptor function,” Journal of Clinical Investigation, vol. 108, no. 1, pp. 25–30, 2001.
- R. A. Coleman, W. L. Smith, and S. Narumiya, “VIII. International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes,” Pharmacological Reviews, vol. 46, no. 2, pp. 205–229, 1994.
- H. Hirai, K. Tanaka, O. Yoshie et al., “Prostaglandin D2 selectively induces chemotaxis in T helper type 2 cells, eosinophils, and basophils via seven-transmembrane receptor CRTH2,” Journal of Experimental Medicine, vol. 193, no. 2, pp. 255–261, 2001.
- G. Monneret, S. Gravel, M. Diamond, J. Rokach, and W. S. Powell, “Prostaglandin D2 is a potent chemoattractant for human eosinophils that acts via a novel DP receptor,” Blood, vol. 98, no. 6, pp. 1942–1948, 2001.
- K. Nagata, H. Hirai, K. Tanaka et al., “CRTH2, an orphan receptor of T-helper-2-cells, is expressed on basophils and eosinophils and responds to mast cell-derived factor(s),” FEBS Letters, vol. 459, no. 2, pp. 195–199, 1999.
- M. Bhattacharya, K. G. Peri, G. Almazan et al., “Nuclear localization of prostaglandin E2 receptors,” Proceeding of the National Academy of Science USA, vol. 22, no. 95, pp. 15792–15797, 1998.
- L. C. Bell-Parikh, T. Ide, J. A. Lawson, P. McNamara, M. Reilly, and G. A. FitzGerald, “Biosynthesis of 15-deoxy--PGJ2 and the ligation of PPARγ,” Journal of Clinical Investigation, vol. 112, no. 6, pp. 945–955, 2003.
- M. P. Pereira, O. Hurtado, A. Cárdenas et al., “Rosiglitazone and 15-deoxy--prostaglandin J2 cause potent neuroprotection after experimental stroke through noncompletely overlapping mechanisms,” Journal of Cerebral Blood Flow and Metabolism, vol. 26, no. 2, pp. 218–229, 2006.
- W. J. Kim, J. H. Kim, and S. K. Jang, “Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A,” EMBO Journal, vol. 26, no. 24, pp. 5020–5032, 2007.
- M. Juzan, I. Hostein, and N. Gualde, “Role of thymus-eicosanoids in the immune response,” Prostaglandins Leukotrienes and Essential Fatty Acids, vol. 46, no. 4, pp. 247–255, 1992.
- N. Gualde and H. Harizi, “Prostanoids and their receptors that modulate dendritic cell-mediated immunity,” Immunology and Cell Biology, vol. 82, no. 4, pp. 353–360, 2004.
- L. Yang, N. Yamagata, R. Yadav et al., “Cancer-associated immunodefidency and dendritic cell abnormalities mediated by the prostaglandin EP2 receptor,” Journal of Clinical Investigation, vol. 111, no. 5, pp. 727–735, 2003.
- R. Pettipher, T. T. Hansel, and R. Armer, “Antagonism of the prostaglandin D2 receptors DP1 and CRTH2 as an approach to treat allergic diseases,” Nature Reviews Drug Discovery, vol. 6, no. 4, pp. 313–325, 2007.
- R. Pettipher, “The roles of the prostaglandin D2 receptors DP1 and CRTH2 in promoting allergic responses,” British Journal of Pharmacology, vol. 153, no. 1, pp. S191–S199, 2008.
- M. N. Ajuebor, A. Singh, and J. L. Wallace, “Cyclooxygenase-2-derived prostaglandin D2 is an early anti-inflammatory signal in experimental colitis,” American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 279, no. 1, pp. G238–G244, 2000.
- A. Ianaro, A. Ialenti, P. Maffia, B. Pisano, and M. Di Rosa, “Role of cyclopentenone prostaglandins in rat carrageenin pleurisy,” FEBS Letters, vol. 508, no. 1, pp. 61–66, 2001.
- A. Arimura, K. Yasui, J. Kishino et al., “Prevention of allergic inflammation by a novel prostaglandin receptor antagonist, S-5751,” Journal of Pharmacology and Experimental Therapeutics, vol. 298, no. 2, pp. 411–419, 2001.
- J. Banchereau and R. M. Steinman, “Dendritic cells and the control of immunity,” Nature, vol. 392, no. 6673, pp. 245–252, 1998.
- K. Shortman and S. H. Naik, “Steady-state and inflammatory dendritic-cell development,” Nature Reviews Immunology, vol. 7, no. 1, pp. 19–30, 2007.
- H. Harizi, “Reciprocal crosstalk between dendritic cells and natural killer cells under the effects of PGE2 in immunity and immunopathology,” Cellular and Molecular Immunology, vol. 10, no. 3, pp. 213–221, 2013.
- D. M. Holt, X. Ma, N. Kundu, P. D. Collin, and A. M. Fulton, “Modulation of host natural killer cell functions in breast cancer via prostaglandin E2 receptors EP2 and EP4,” Journal of Immunotherapy, vol. 35, no. 2, pp. 179–188, 2012.
- M. Vitale, M. D. Chiesa, S. Carlomagno et al., “The small subset of CD56brightCD16- natural killer cells is selectively responsible for both cell proliferation and interferon-γ production upon interaction with dendritic cells,” European Journal of Immunology, vol. 34, no. 6, pp. 1715–1722, 2004.
- F. Gerosa, B. Baldani-Guerra, C. Nisii, V. Marchesini, G. Carra, and G. Trinchieri, “Reciprocal activating interaction between natural killer cells and dendritic cells,” Journal of Experimental Medicine, vol. 195, no. 3, pp. 327–333, 2002.
- N. C. Fernandez, A. Lozier, C. Flament et al., “Dendritic cells directly trigger NK cell functions: cross-talk relevant in innate anti-tumor immune responses in vivo,” Nature Medicine, vol. 5, no. 4, pp. 405–411, 1999.
- M. A. Degli-Esposti and M. J. Smyth, “Close encounters of different kinds: dendritic cells and NK cells take centre stage,” Nature Reviews Immunology, vol. 5, no. 2, pp. 112–124, 2005.
- W. Walker and D. Rotondo, “Prostaglandin E2 is a potent regulator of interleukin-12- and interleukin-18-induced natural killer cell interferon-γ synthesis,” Immunology, vol. 111, no. 3, pp. 298–305, 2004.
- L. Martinet, C. Jean, G. Dietrich, J.-J. Fournié, and R. Poupot, “PGE2 inhibits natural killer and γδ T cell cytotoxicity triggered by NKR and TCR through a cAMP-mediated PKA type I-dependent signaling,” Biochemical Pharmacology, vol. 80, no. 6, pp. 838–845, 2010.
- D. Holt, X. Ma, N. Kundu, and A. Fulton, “Prostaglandin E2 (PGE2) suppresses natural killer cell function primarily through the PGE2 receptor EP4,” Cancer Immunology, Immunotherapy, vol. 60, no. 11, pp. 1577–1586, 2011.
- K. Gustafsson, M. Ingelsten, L. Bergqvist, J. Nyström, B. Andersson, and A. Karlsson-Parra, “Recruitment and activation of natural killer cells in vitro by a human dendritic cell vaccine,” Cancer Research, vol. 68, no. 14, pp. 5965–5971, 2008.
- A. H. Enk, V. L. Angeloni, M. C. Udey, and S. I. Katz, “Inhibition of Langerhans cell antigen-presenting function by IL-10: a role for IL-10 in induction of tolerance,” Journal of Immunology, vol. 151, no. 5, pp. 2390–2398, 1993.
- A. H. Enk, H. Jonuleit, J. Saloga, and J. Knop, “Dendritic cells as mediators of tumor-induced tolerance in metastatic melanoma,” International Journal of Cancer, vol. 4, no. 73, pp. 309–316, 1997.
- S. Mandaric, S. M. Walton, T. R. Rülicke et al., “IL-10 suppression of NK/DC crosstalk leads to poor priming of MCMV-specific CD4 T cells and prolonged MCMV persistence,” PLoS Pathogenes, vol. 8, no. 8, Article ID 1002846, 2012.
- A. J. Troy, K. L. Summers, P. J. T. Davidson, C. H. Atkinson, and D. N. J. Hart, “Minimal recruitment and activation of dendritic cells within renal cell carcinoma,” Clinical Cancer Research, vol. 4, no. 3, pp. 585–593, 1998.
- S. Józefowski, M. Bobek, and J. Marcinkiewicz, “Exogenous but not endogenous prostanoids regulate cytokine secretion from murine bone marrow dendritic cells: EP2, DP, and IP but not EP1, EP3, and FP prostanoid receptors are involved,” International Immunopharmacology, vol. 3, no. 6, pp. 865–878, 2003.
- C. Nataraj, D. W. Thomas, S. L. Tilley et al., “Receptors for prostaglandin E2 that regulate cellular immune responses in the mouse,” Journal of Clinical Investigation, vol. 108, no. 8, pp. 1229–1235, 2001.
- P. Singh, J. Hoggatt, P. Hu et al., “Blockade of prostaglandin E2 signaling through EP1 and EP3 receptors attenuates Flt3L-dependent dendritic cell development from hematopoietic progenitor cells,” Blood, vol. 119, no. 7, pp. 1671–1682, 2012.
- Y. Urade and O. Hayaishi, “Prostaglandin D synthase: structure and function,” Vitamins and Hormones, vol. 58, pp. 89–120, 2000.
- T. Matsuoka, M. Hirata, H. Tanaka et al., “Prostaglandin D2 as a mediator of allergic asthma,” Science, vol. 287, no. 5460, pp. 2013–2017, 2000.
- C. Robinson, C. C. Hardy, and S. T. Holgate, “Pulmonary synthesis, release, and metabolism of prostaglandins,” Journal of Allergy and Clinical Immunology, vol. 76, pp. 265–271, 1985.
- J. J. Murray, A. B. Tonnel, and A. R. Brash, “Release of prostaglandin D2 into human airways during acute antigen challenge,” The New England Journal of Medicine, vol. 315, no. 13, pp. 800–804, 1986.
- H. Sandig, J. E. Pease, and I. Sabroe, “Contrary prostaglandins: the opposing roles of PGD2 and its metabolites in leukocyte function,” Journal of Leukocyte Biology, vol. 81, no. 2, pp. 372–382, 2007.
- P. Gosset, F. Bureau, V. Angeli et al., “Prostaglandin D2 affects the maturation of human monocyte-derived dendritic cells: consequence on the polarization of naive Th cells,” Journal of Immunology, vol. 170, no. 10, pp. 4943–4952, 2003.
- M. Jira, E. Antosova, V. Vondra, J. Strejcek, H. Mazakova, and J. Prazakova, “Natural killer and interleukin-2 induced cytotoxicity in asthmatics. I. Effect of acute antigen-specific challenge,” Allergy, vol. 43, no. 4, pp. 294–298, 1988.
- Y. Chen, B. Perussia, and K. S. Campbell, “Prostaglandin D2 suppresses human NK cell function via signaling through D prostanoid receptor,” Journal of Immunology, vol. 179, no. 5, pp. 2766–2773, 2007.
- G. Theiner, A. Gessner, and M. B. Lutz, “The mast cell mediator PGD2 suppresses IL-12 release by dendritic cells leading to TH2 polarized immune responses in vivo,” Immunobiology, vol. 211, no. 6-8, pp. 463–472, 2006.
- K. Kabashima, D. Sakata, M. Nagamachi, Y. Miyachi, K. Inaba, and S. Narumiya, “Prostaglandin E2-EP4 signaling initiates skin immune responses by promoting migration and maturation of Langerhans cells,” Nature Medicine, vol. 9, no. 6, pp. 744–749, 2003.
- K. Steinbrink, M. Wölfl, H. Jonuleit, J. Knop, and A. H. Enk, “Induction of tolerance by IL-10-treated dendritic cells,” Journal of Immunology, vol. 159, no. 10, pp. 4772–4780, 1997.
- D. Braun, R. S. Longman, and M. L. Albert, “A two-step induction of indoleamine 2,3 dioxygenase (IDO) activity during dendritic-cell maturation,” Blood, vol. 106, no. 7, pp. 2375–2381, 2005.
- A. L. Mellor and D. H. Munn, “IDO expression by dendritic cells: tolerance and tryptophan catabolism,” Nature Reviews Immunology, vol. 4, no. 10, pp. 762–774, 2004.
- F. G. M. Snijdewint, P. Kalinski, E. A. Wierenga, J. D. Bos, and M. L. Kapsenberg, “Prostaglandin E2 differentially modulates cytokine secretion profiles of human T helper lymphocytes,” Journal of Immunology, vol. 150, no. 12, pp. 5321–5329, 1993.
- Y. Urade, M. Ujihara, Y. Horiguchi, K. Ikai, and O. Hayaishi, “The major source of endogenous prostaglandin D2 production is likely antigen-presenting cells. Localization of glutathione-requiring prostaglandin D synthetase in histiocytes, dendritic, and Kupffer cells in various rat tissues,” Journal of Immunology, vol. 143, no. 9, pp. 2982–2989, 1989.
- K. Tanaka, K. Ogawa, K. Sugamura, M. Nakamura, S. Takano, and K. Nagata, “Cutting edge: differential production of prostaglandin D2 by human helper T cell subsets,” Journal of Immunology, vol. 164, no. 5, pp. 2277–2280, 2000.
- N. Bilyk and P. G. Holt, “Cytokine modulation of the immunosuppressive phenotype of pulmonary alveolar macrophage populations,” Immunology, vol. 86, no. 2, pp. 231–237, 1995.
- I. Cebola and M. A. Peinado, “Epigenetic deregulation of the COX pathway in cancer,” Progress in Lipid Research, vol. 51, no. 4, pp. 301–313, 2012.
- S. G. Gray, N. Al-Sarraf, A.-M. Baird, M.-C. Cathcart, E. McGovern, and K. J. O'Byrne, “Regulation of EP receptors in non-small cell lung cancer by epigenetic modifications,” European Journal of Cancer, vol. 45, no. 17, pp. 3087–3097, 2009.
- B. G. Cosío, B. Mann, K. Ito et al., “Histone acetylase and deacetylase activity in alveolar macrophages and blood mononocytes in asthma,” American Journal of Respiratory and Critical Care Medicine, vol. 170, no. 2, pp. 141–147, 2004.
- Y. Sugino, A. Misawa, J. Inoue et al., “Epigenetic silencing of prostaglandin E receptor 2 (PTGER2) is associated with progression of neuroblastomas,” Oncogene, vol. 26, no. 53, pp. 7401–7413, 2007.