686549.fig.004a
(a)
686549.fig.004b
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686549.fig.004c
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Figure 4: Cross-reactivity to variant M2e peptides. The cross-reactivity of immune sera to variant M2e peptides was assessed by ELISA. G1: Soluble tetrameric M2e protein (stM2e), G2: tM2e VLPs; G3: tM2e/tFliC VLPs; G4: tM2e VLP + tFliC VLPs; G5: tM2e VLP + soluble flagellin; G6: soluble flagellin; NC: naive group. (a) Serum IgG; (b) IgG1; (c) IgG2a. Each group has 6 mice. The asterisk (*) indicates a significant difference between G2 and G1, G3 and G1, G4 and G1, and G5 and G1. The “+” indicates a significant difference between G3 and G2, G4 and G2, and G5 and G2.