Preliminary Results of Implantation in Animal Model and Osteoblast Culture Evaluation of Prototypes of Biomimetic Multispiked Connecting Scaffold for Noncemented Stemless Resurfacing Hip Arthroplasty Endoprostheses
Figure 14
(a, b) Fluorescence microscopy after acridine orange (AO) staining of human osteoblasts ((NHOst) Lonza) after cultivation of the biomimetic MSC-Scaffold preprototypes for 10 days. Cells adhered to MSC-Scaffold’s spikes and filled the space between them; they spread on the surface of the biomimetic MSC-Scaffold and start to contact each other via cytoplasmic extensions (white arrows) creating a three-dimensional cell-to-cell network. Thus, the preprototype spikes (mimicking the interdigitations of periarticular subchondral bone) can indeed act like a scaffold for osteoblasts.
