Table 1: Experimental design for in vitro pyrogen testing.

Observation periods (days) Fresh blood (single donor) (hrs) Fresh lymphocytes (h) (single donor) Pooled lymphocytes cryopreserved in DMSO (h) Pooled lymphocytes cryopreserved in Glycerol (h)
Pyrogens
LPS (5 EU)LTA (1  g/ L)LPS (5 EU)LTA (1  g/ L)LPS (5 EU)LTA (1  g/ L)LPS (5 EU)LTA (1  g/ L)

0 7 5 and 7 5 and 6 50000
753 and 434
143343
303334
603433